Purification and Characterization of Antimicrobial Agent from Streptomyces sp. JF 714876
DOI:
https://doi.org/10.37285/ijpsn.2020.13.3.9Abstract
Antibacterial agents are essential drugs for combating the bacterial infections. In this study, the potent actinomycetes strain active against pathogenic bacteria and fungi was isolated from Solapur region of Maharashtra. Based on morphological, physiological, biochemical and 16S rRNA sequence, isolate was identified as Streptomyces sp. JF714876. The nutritional requirement and production condition were optimized to increase the antibiotic production. The crude compound from optimized media was extracted using ethyl acetate at pH -3, the active fraction was purified and separated using silica gel column chromatography and thin layer chromatography with Rf value 0.8. The MIC of active compound tested against Aspergillus species was recorded at 40 µg/ml whereas against dermatophytes at 50 µg/ml concentration. Physicochemical characteristic of bioactive agent indicate that the compound belongs to alkaloid group.
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Keywords:
Streptomyces sp. JF714876, Antimicrobial activity, characterization, Purification
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Published
2020-05-31
How to Cite
1.
G.M V, Babanagare S, Kamradgi S. Purification and Characterization of Antimicrobial Agent from Streptomyces sp. JF 714876 . Scopus Indexed [Internet]. 2020 May 31 [cited 2024 May 22];13(3):4952-9. Available from: https://www.ijpsnonline.com/index.php/ijpsn/article/view/934
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Research Articles
References
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Al-ghazali LH and Omran R (2017). Optimization of medium composition for antibacterial metabolite production from streptomyces sp. Asian J Pharm Clin Res 10(9): 381-385.
Anuradha S, Shravan Kumar K, Bhama S and Kishan V (2016). Fermentatiin, isolation, purification and characterization of antitubarcular antibiotic from Streptomyces luridus MTCC4402. Ind J Exp Biol 54: 577-585.
Atta HM, Bayoumi R, El-Sehrawi M, Aboshady A and Al-Humiany A (2010a). Tunicamysin antibiotic production by Streptomyces torulosus KH4; Fermentation, purification and biological activity. African J Basic & Appl Sci 2: 177-183.
Atta HM, El-Sehrawi MH and Bahobail AS (2011a). Antifungal macrodiode production by Streptomyces albidoflavus-143: fermentation, purification and biological activities. J Am Sci 7(3): 13-22.
Augustine SK, Bhavsar SP and Kapadnis BP (2005). Production of growth dependent metabolite active against dermatophytes by Streptomyces rochei AK 39. Indian J Med Res 121: 164-170.
Brewster Q, Calvin AV and William EM (1977). Unitized experiments in organic chemistry 4th, Van Nostrand Company, New York.
El-Mehalawy AA, Abd-Allah NA, Mohamed RM and Abu-Shady MR (2005). Actinomycetes antagonizing plant and human pathogenic fungi. II. factors affecting antifungal production and chemical characterization of the active components. International J Agriculture and Biol 7(2): 188-196.
El-Nagger MY, El-Assar SA and Abdul-Gawad SM (2006). Meroparamycin production by newly isolated Streptomyces sp. strain MAR01: Taxonomy, fermentation, purification and structural elucidation. J Microbiol 44: 432-438.
Gaurav VS, Dipak G, Subhash G, Riddhi L, Niketa D, Prashant DK, Devendra JV and Gaurav SD (2014). Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot, India. Biotechnology Reports 1-2: 2-7
Gottlieb D (1961). An evolution of criterion and procedure used in the description and characterization of Streptomyces, A co-operative study. Appl Microbiol 9: 55-60.
Hall TA (1999). Bioedit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic acid Symp Ser 41: 95-98.
Holding AJ and Collee JG (1971). Routine biochemical tests. Methods Microbiol. 6A: 1-34.
Holt JG, Krieg N, Sneath PAH and Williams ST (1994). Bergey’s manual of determinatie bacteriology. 9th Edn., Williams and Wilkins Co., Baltimore, USA.
Kaur H, Arora DS and Sharma V (2014) Isolation, purification, and characterization of antimicrobial compound 6-[1, 2-dimethyl-6-(2-methyl-allyloxy)-hexyl]-3-(2- methoxy-phenyl)-chromen- 4-one from Penicilliumsp. HT-28. Appl Biochem Biotechnol 173(8): 1963-76.
Kavitha A, Prabhakar P, Vijayalakshmi M, Venkateswarlu Y (2010). Purification and biological evaluation of the metabolites produced by Streptomyces sp. TK-VL_333. Res Microbiol 61: 335-45.
Kotake C, Yamasaki T, Moriyama T, Shinoda M, Komiyama N, Furumai T, Konishi and Oki T (1992). Butyrolactols A and B, new antifungal antibiotics. Taxonomy, isolation, physico chemical properties, structure and biological activity. J Antibiotic 45: 1442-1450.
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Kumar S and Kannbiran K (2010). Diversity and optimization of process parameters for the growth of Streptomyces VITSVK9 sp. isolated from Bay of Bengal, India. Indian J Nat Env Sci 1(2): 56-65.
Oskay M (2011). Isolation and purification of two metabolites (KGG32-A and KGG32-B) from soil bacterium, Streptomyces sp. KGG32. Int J Agric Biol 13: 369-374.
Pallaa MS, Guntukua GS, Muthyalaa MK , Pingalia S, and Prafulla K (2018). Isolation and molecular characterization of antifungal metabolite producing actinomycete from mangrove soil. University Journal of Basic and Applied Sciences 7: 250–256
Pathalam G, Appadurai DR, Rajendran AD, Munusamy RG, Michael GP, Naif AA and Savarimuthu I (2017). Antimicrobial activity of some actinomycetes from Western Ghats of Tamil Nadu, India Alexandria Journal of Medicine 53: 101-110
Pudi N, Varikuti G, Badana A, Gavara MM, Kumari S, and Malla R (2016). Studies on Optimization of Growth Parameters for Enhanced Production of Antibiotic Alkaloids by Isolated Marine actinomycetes. J Appli Pharmaceutical Science 6(10): 181-188.
Rajeswari P, Jose PA, Amiya R, and Jebakumar SR (2015). Characterization of saltern based Streptomyces sp. and statistical media optimization for its improved antibacterial activity. Front Microbiol Jan 21.
https://doi.org/10.3389/fmicb.2014.00753 PMID: 25653640
Ripa FA, Nikkon F, Zaman and Khondkar P (2009). Optimal Conditions for Antimicrobial Metabolites Production from a New Streptomyces sp. RUPA-08PR Isolated from Bangladeshi Soil. Mycobiology 37: 211-14.
Selvin J, Joseph S, Asha KRT, Manjusha WA, Sangeetha VS, Jayaseema DM, Antony MC and Denslin Vinitha AJ (2004). Antibacterial potential of antagonistic Streptomyces sp. isolated from marine sponge Dendrilla nigra. Microbiol Ecol 50: 117-122.
Shan W, Zhou Y, Liu H and Yu X (2018). Endophytic Actinomycetes from Tea Plants (Camellia sinensis): Isolation, Abundance, Antimicrobial, and Plant-Growth-Promoting Activities. BioMed Research International Volume 2018.
https://doi.org/10.1155/2018/1470305.
Sharma M and Manhas RK (2019). Purification and characterization of actinomycins from Streptomyces strain M7 active against methicillin resistant Staphylococcus aureus and vancomycin resistant Enterococcus. BMC microbiol 19: 44.
Shirling EB and Gottlieb D (1966). Methods for characterization of Streptomyces sp. Int J Sys Bacteriol 16: 313-340.
Souagui S, Djoudi W, Boudries H, Bechet M, Leclere V and Kecha K (2019). Modeling and Statistical Optimization of Culture Conditions for Improvement of Antifungal Compounds Production by Streptomyces albidoflavus S19 Strain of Wastewater Origin. Anti-Infective Agents 17: 39-49.
Tresner HD, Hayes JA and Backns EJ (1966). Differential tolerance of Streptomyces to sodium chloride as a taxonomic aid. Appl Microbial 16: 1134-1136.
Vasavada SH, Thumar JT and SinghSP (2006). Secretion of a potential antibiotic by self tolerant and alkaliphilic Streptomyces sannanensin strain RJT 1. Curr Sci 91: 393-397.
Vidyasagar GM, Shankaravva B, Begum R, Imrose S, Sagar R and Raibagkar RL (2012). Antimicrobial Activity of Silver Nanoparticles Synthesized by Streptomyces Species JF714876. Int J Pharm Sci Nanotechl 5(1): 1638-1642
Williams ST and Cross T (1971). Isolation, purification, cultivation and preservation of actinomycetes. Methods Microbiol 4: 295-334.
William JG and Sherman N (1999). Microbiology, A laboratory manual (Harlow: Benjamin) pp 263-264.
Yassin MH, Ghaly MF, Shash SM and El-Doraidy EH (2005). Potentiality of actinomycetes isolates against dermatophytic fungi. 1-Biological, taxonomical studies and environmental factors affecting the antifungal production of Streptomyces kanamyceticus EHE-68. J Microbiol Egypt 11: 45-62.
Yun TY, Feng RJ, Zhou DB, Pan YY, Chen YF, and Wang F (2018). Optimization of fermentation conditions through response surface methodology for enhanced antibacterial metabolite production by Streptomyces sp. 1-14 from cassava rhizosphere. PLoS ONE 13(11): e0206497
Zhou Y, Sun Y, He H, Feng J, Zhang X and Han L (2017). Optimization of medium compositions to improve a novel glycoprotein production by Streptomyces kanasenisi ZX01. AMB Express 7: 6-14.
Ahsan T, Chen J, Wu Y and Irfan M (2017). Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460. AMB Expr 7: 96.
Al-ghazali LH and Omran R (2017). Optimization of medium composition for antibacterial metabolite production from streptomyces sp. Asian J Pharm Clin Res 10(9): 381-385.
Anuradha S, Shravan Kumar K, Bhama S and Kishan V (2016). Fermentatiin, isolation, purification and characterization of antitubarcular antibiotic from Streptomyces luridus MTCC4402. Ind J Exp Biol 54: 577-585.
Atta HM, Bayoumi R, El-Sehrawi M, Aboshady A and Al-Humiany A (2010a). Tunicamysin antibiotic production by Streptomyces torulosus KH4; Fermentation, purification and biological activity. African J Basic & Appl Sci 2: 177-183.
Atta HM, El-Sehrawi MH and Bahobail AS (2011a). Antifungal macrodiode production by Streptomyces albidoflavus-143: fermentation, purification and biological activities. J Am Sci 7(3): 13-22.
Augustine SK, Bhavsar SP and Kapadnis BP (2005). Production of growth dependent metabolite active against dermatophytes by Streptomyces rochei AK 39. Indian J Med Res 121: 164-170.
Brewster Q, Calvin AV and William EM (1977). Unitized experiments in organic chemistry 4th, Van Nostrand Company, New York.
El-Mehalawy AA, Abd-Allah NA, Mohamed RM and Abu-Shady MR (2005). Actinomycetes antagonizing plant and human pathogenic fungi. II. factors affecting antifungal production and chemical characterization of the active components. International J Agriculture and Biol 7(2): 188-196.
El-Nagger MY, El-Assar SA and Abdul-Gawad SM (2006). Meroparamycin production by newly isolated Streptomyces sp. strain MAR01: Taxonomy, fermentation, purification and structural elucidation. J Microbiol 44: 432-438.
Gaurav VS, Dipak G, Subhash G, Riddhi L, Niketa D, Prashant DK, Devendra JV and Gaurav SD (2014). Isolation and partial purification of erythromycin from alkaliphilic Streptomyces werraensis isolated from Rajkot, India. Biotechnology Reports 1-2: 2-7
Gottlieb D (1961). An evolution of criterion and procedure used in the description and characterization of Streptomyces, A co-operative study. Appl Microbiol 9: 55-60.
Hall TA (1999). Bioedit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic acid Symp Ser 41: 95-98.
Holding AJ and Collee JG (1971). Routine biochemical tests. Methods Microbiol. 6A: 1-34.
Holt JG, Krieg N, Sneath PAH and Williams ST (1994). Bergey’s manual of determinatie bacteriology. 9th Edn., Williams and Wilkins Co., Baltimore, USA.
Kaur H, Arora DS and Sharma V (2014) Isolation, purification, and characterization of antimicrobial compound 6-[1, 2-dimethyl-6-(2-methyl-allyloxy)-hexyl]-3-(2- methoxy-phenyl)-chromen- 4-one from Penicilliumsp. HT-28. Appl Biochem Biotechnol 173(8): 1963-76.
Kavitha A, Prabhakar P, Vijayalakshmi M, Venkateswarlu Y (2010). Purification and biological evaluation of the metabolites produced by Streptomyces sp. TK-VL_333. Res Microbiol 61: 335-45.
Kotake C, Yamasaki T, Moriyama T, Shinoda M, Komiyama N, Furumai T, Konishi and Oki T (1992). Butyrolactols A and B, new antifungal antibiotics. Taxonomy, isolation, physico chemical properties, structure and biological activity. J Antibiotic 45: 1442-1450.
Kumar PS, Duraipandiyan V and Ignacimuthu S (2014). Isolation, screening and partial purification of antimicrobial antibiotics from soil Streptomyces sp. SCA 7. Kaohsiung Journal of Medical Sciences 30: 435-446
Kumar S and Kannbiran K (2010). Diversity and optimization of process parameters for the growth of Streptomyces VITSVK9 sp. isolated from Bay of Bengal, India. Indian J Nat Env Sci 1(2): 56-65.
Oskay M (2011). Isolation and purification of two metabolites (KGG32-A and KGG32-B) from soil bacterium, Streptomyces sp. KGG32. Int J Agric Biol 13: 369-374.
Pallaa MS, Guntukua GS, Muthyalaa MK , Pingalia S, and Prafulla K (2018). Isolation and molecular characterization of antifungal metabolite producing actinomycete from mangrove soil. University Journal of Basic and Applied Sciences 7: 250–256
Pathalam G, Appadurai DR, Rajendran AD, Munusamy RG, Michael GP, Naif AA and Savarimuthu I (2017). Antimicrobial activity of some actinomycetes from Western Ghats of Tamil Nadu, India Alexandria Journal of Medicine 53: 101-110
Pudi N, Varikuti G, Badana A, Gavara MM, Kumari S, and Malla R (2016). Studies on Optimization of Growth Parameters for Enhanced Production of Antibiotic Alkaloids by Isolated Marine actinomycetes. J Appli Pharmaceutical Science 6(10): 181-188.
Rajeswari P, Jose PA, Amiya R, and Jebakumar SR (2015). Characterization of saltern based Streptomyces sp. and statistical media optimization for its improved antibacterial activity. Front Microbiol Jan 21.
https://doi.org/10.3389/fmicb.2014.00753 PMID: 25653640
Ripa FA, Nikkon F, Zaman and Khondkar P (2009). Optimal Conditions for Antimicrobial Metabolites Production from a New Streptomyces sp. RUPA-08PR Isolated from Bangladeshi Soil. Mycobiology 37: 211-14.
Selvin J, Joseph S, Asha KRT, Manjusha WA, Sangeetha VS, Jayaseema DM, Antony MC and Denslin Vinitha AJ (2004). Antibacterial potential of antagonistic Streptomyces sp. isolated from marine sponge Dendrilla nigra. Microbiol Ecol 50: 117-122.
Shan W, Zhou Y, Liu H and Yu X (2018). Endophytic Actinomycetes from Tea Plants (Camellia sinensis): Isolation, Abundance, Antimicrobial, and Plant-Growth-Promoting Activities. BioMed Research International Volume 2018.
https://doi.org/10.1155/2018/1470305.
Sharma M and Manhas RK (2019). Purification and characterization of actinomycins from Streptomyces strain M7 active against methicillin resistant Staphylococcus aureus and vancomycin resistant Enterococcus. BMC microbiol 19: 44.
Shirling EB and Gottlieb D (1966). Methods for characterization of Streptomyces sp. Int J Sys Bacteriol 16: 313-340.
Souagui S, Djoudi W, Boudries H, Bechet M, Leclere V and Kecha K (2019). Modeling and Statistical Optimization of Culture Conditions for Improvement of Antifungal Compounds Production by Streptomyces albidoflavus S19 Strain of Wastewater Origin. Anti-Infective Agents 17: 39-49.
Tresner HD, Hayes JA and Backns EJ (1966). Differential tolerance of Streptomyces to sodium chloride as a taxonomic aid. Appl Microbial 16: 1134-1136.
Vasavada SH, Thumar JT and SinghSP (2006). Secretion of a potential antibiotic by self tolerant and alkaliphilic Streptomyces sannanensin strain RJT 1. Curr Sci 91: 393-397.
Vidyasagar GM, Shankaravva B, Begum R, Imrose S, Sagar R and Raibagkar RL (2012). Antimicrobial Activity of Silver Nanoparticles Synthesized by Streptomyces Species JF714876. Int J Pharm Sci Nanotechl 5(1): 1638-1642
Williams ST and Cross T (1971). Isolation, purification, cultivation and preservation of actinomycetes. Methods Microbiol 4: 295-334.
William JG and Sherman N (1999). Microbiology, A laboratory manual (Harlow: Benjamin) pp 263-264.
Yassin MH, Ghaly MF, Shash SM and El-Doraidy EH (2005). Potentiality of actinomycetes isolates against dermatophytic fungi. 1-Biological, taxonomical studies and environmental factors affecting the antifungal production of Streptomyces kanamyceticus EHE-68. J Microbiol Egypt 11: 45-62.
Yun TY, Feng RJ, Zhou DB, Pan YY, Chen YF, and Wang F (2018). Optimization of fermentation conditions through response surface methodology for enhanced antibacterial metabolite production by Streptomyces sp. 1-14 from cassava rhizosphere. PLoS ONE 13(11): e0206497
Zhou Y, Sun Y, He H, Feng J, Zhang X and Han L (2017). Optimization of medium compositions to improve a novel glycoprotein production by Streptomyces kanasenisi ZX01. AMB Express 7: 6-14.
