Screening and Estimation of Secondary Metabolites in Leaf Extracts of Delonix elata (L.) Gamble and their Inhibitory Activities against Pathogens and Oxidative Stress
DOI:
https://doi.org/10.37285/ijpsn.2020.13.3.5Abstract
Delonix elata (L.) Gamble is a tree commonly available and well known to be used for joint pain, flatulence and arthritic disorders. The main objective of this study was to investigate biological activities of the phytochemicals of leaf extracts of Delonix elata (L.) for activities against pathogens and oxidative stress. The plant source was sequentially extracted by the following solvents chloroform, ethyl acetate, acetone, methanol and water. Antibacterial activity was tested against six pathogens with disc diffusion method; MIC and MBC were also done by double dilution method. The standard procedures were followed for estimation of flavonoids, flavanones, phenolic compounds and alkaloids. Antioxidant potential was determined by DPPH assay. Obtained five different solvent extracts and observed clear zone of inhibitions against all the pathogens on the extract of ethyl acetate. The MIC and MBC results were observed against all the pathogens, compared to other pathogens Staphyl-ococcus aureus inhibits at very low concentration (30mg/mL) of the extract. The rich source of flavonoids and phenolic compounds present in aqueous extract, flavanones present in methanol extract and alkaloids present in ethyl acetate extract. As the antioxidant assay, higher percentage (82.82%) of inhibition was calculated in ethyl acetate extract. On phytochemical screening the presence of phenols, flavonoids, alkaloids, steroids, terpenoids, carbohydrates, proteins and aminoacids were observed. This investigation shows the potential inhibitory activity of ethyl acetate extract of the plant leaves. The phytoconstituents are rich sources present in the different solvent extracts of D. elata leaf that may be responsible for the potential antimicrobial and antioxidant activity. S. aureus is one of the leading causes of infection in hospital. Here we found that D. elata leaf extract inhibit the pathogen S. aureus at very low concentration (30mg/mL) itself. Therefore, it is suggested that these leaves are helpful for identification of new drugs against this pathogen.
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Delonix elata, Phytochemical screening, Antibacterial activity, Total phenolic compounds, Total antioxidant activity
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