Isolation and Determination of the Major Principle or Causative Agent behind the 2016 Published Break through Discovery of Dr. M.S. Reddy’s “Multiple Mixed Strain Probiotic Therapy”, in Successfully Treating the Lethal Hospital Acquired Infections Due to

DOI:

https://doi.org/10.37285/ijpsn.2016.9.6.7

Authors

  • Malireddy S Reddy
  • D.R.K Reddy

Abstract

A multiple mixed strain Probiotic culture was compounded using several naturally antibiotic resistant beneficial microorganisms belonging to different genera and species, along with their bacteriocins and growth end products or metabolites. Prior to mixing several individually grown single strains of Probiotics, compatibility studies were conducted to eliminate the strain dominance in the mixed cultures using direct differential plating techniques and strain specific bacteriophages,using the procedures  outlined in our earlier publication. The multiple mixed stain Probiotics thus prepared were studied to see weather Probiotic bacteria by themselves or their bacteriocins and other end products of growth (without the live Probiotics) or the combination of live Probiotics and their bacteriocins and other growth end products, were responsible to inhibit the growth and proliferation of the lethal hospital acquired infections i.e., Clostridium difficile (C. diff) and Methicillin Resistant Staphylococcus aureus (MRSA). In addition, several procedures of preparing the functional mixed strain Probiotics, using liquid nitrogen freezing and freeze drying (lyophilization)were evaluated,tocomeupwiththebest

procedure suitable to maximize their subsequent inhibitory effect on C. diff and MRSA, both under the laboratory conditions and in the practical clinical hospital conditions. Community based clinical trials were conducted to check the effect of multiple mixed strain Probiotics with and without their bacteriocins and their other growth end products, to cure the lethalC. diff infection under practical hospital conditions. The results revealed and confirmed that our 2016 novel discovery is still the best way to prepare and administer the multiple mixed strain Probiotics to treat the lethal hospital acquired infections. Also this study reconfirmed that the liquid nitrogen freezing is far superior to freeze drying or lyophilization to preserve the maximum efficiency of multiple mixed strain Probiotics. In conclusion, it proved that even the concentrated high bacterial cell number multiple mixed strain Probiotics (without their bacteriocins and other growth metabolites) are not as effective compared to the multiple mixed strain Probiotics frozen using liquid nitrogen, along with their bacteriocins and growth metabolites, to cure the lethal C. diff infection under practical clinical hospital conditions.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Keywords:

Probiotics, bacteriocins, MRSA, C. diff, multiple mixed strain Probiotic therapy, Multiple mixed strain Probiotics, Nosocomial infections, Hospital acquired infections, Natural antibiotic resistant Probiotics, Propionibacterium, lactic acid producing Probiotic bacteria

Downloads

Published

2016-11-30

How to Cite

1.
Reddy MS, Reddy D. Isolation and Determination of the Major Principle or Causative Agent behind the 2016 Published Break through Discovery of Dr. M.S. Reddy’s “Multiple Mixed Strain Probiotic Therapy”, in Successfully Treating the Lethal Hospital Acquired Infections Due to . Scopus Indexed [Internet]. 2016 Nov. 30 [cited 2024 Dec. 11];9(6):3556-6. Available from: https://www.ijpsnonline.com/index.php/ijpsn/article/view/894

Issue

Section

Research Articles

References

Code of Federal Regulations of Food and Drug (2001). Published by Office of the Federal Register, National Archives and Records Administration, U.S. Government printing office, Washington, D.C., U.S.A.

Fuller R (1989). Probiotics in man and animals. J Appl. Bacterial. 66:365-378.

Holo H, Faye T, Brede DA, Nilsen T, Oregard I and Langsrud T (2002). Bacteriocins of propionibacteria. Lait. 82: 59-68.

Jan G, Belzacq S, Haouzi D, Rouault A, Metivier D and Kromer G (2002). Propionibacteria induce apoptosis of colorectal carcinoma cells via short-chain fatty acids acting on mitochondria. Cell Death Differ. 9: 179-88.

Jan G, Leverrier P, Proudi I and Roland N (2002). Survival and beneficial effects of propionibacteria in the human gut. In vivo and in vitro investigations. Lait. 82: 131-144.

Leverrier P, Dimova D, Pichereau V, Auffrey Y, Boyaval P and Jan G (2003). Susceptibility and adaptive response to bile salts in propionibacterium freudenreichii: physiological and proteomic analysis. Appl. Environ Microbiol. 69: 309-3818.

Metchnikoff E, (1907). The prolongation of life. Optimistic studies. London: Butterworth-Heinemann.

Reddy, MS, Reddy DRK, and Prasad NAV (2000). Herbal and pharmaceutical drugs enhance with Probiotics. U.S. Patent 6,080,401: 1-48.

Reddy MS, and Reddy DRK (2008). Therapeutic effect of multiple mixed strain Probiotics to prevent or cure the hospital associated infections due to Clostridium difficile – C. diff and methicillin resistant Staphylococcus aureus – MRSA. Presented at the 26th annual AAPI medical convention (American Association of Physicians of Indian Origin), June 26-29, Las Vegas, Nevada, USA.

Reddy MS, and Reddy DRK (2009). Probiotic therapy. AAPI Journal, March issue: 28-29.

Reddy MS and Reddy DRK (2007). Probiotics in health and disease vs. Proyurveda (Probiotics L. acidophilus DRK, B. bifidus MS and Prebiotics – Ayurvedic herbs) in health and disease, clinically tested over million human subjects. Presented at 25th (Silver Jubilee) medical convention of AAPI (integrated medicine program on combining Ayurveda into clinical practice), May 9-13, Philadelphia, Pennsylvania, USA.

Reddy MS and Reddy DRK (2011). Anti-aging: Review and Experimental Clinical Study of Bioavailable calcium – Probiotics and their effect on reversing osteopenia, osteoporosis, and other common health conditions. Int J Pharma Sci Nanotech4(3): 1436-1444.

Reddy MS and Reddy DRK (2015). Therapeutic Probiotics for cancer reduction. AAPI Journal, May/June issue.

Reddy MS and Reddy DRK (2016). Development of multiple mixed strain Probiotics for “Probiotic therapy” under clinical conditions, to prevent or cure the deadly hospital acquired infections due to Clostridium difficile (C. diff) and Methicillin resistant staphylococcus aureus (MRSA). Int. J Pharma Sci. Nanotech9:3256-3281.

Reddy MS and Reinbold GW (1972). Effects of some antibiotics and antimicrobial agents on Propionibacterium. J Dairy Sci. 55:665.

Reddy MS, Williams FD and Reinbold GW (1973). Sulfonamide resistance of Propionibacterium: nutrition and transport. Antimicrobial Agents and Chemotherapy 4: 254-258.

Reddy MS, Vedamuthu ER, Washam CJ, and Reinbold GW (1969). Differential agar medium for separating Streptococcus lactis and Streptococcus cremoris. Appl. Microbiol. 198:755-759.

Reddy MS, Vedamuthu ER, Washam CJ and Reinbold GW (1970). Differential agar medium for separating lactic Streptococci and its application to studying strain dominance in starter strains. J Dairy Sci.53:632.

Reddy MS, Vedamuthu ER, Washam CJ and Reinbold GW (1971(a). Associative growth relationship in two strain mixtures of Streptococcus lactis and streptococcus cremoris. J Milk Food Technol. 34:236-240.

Reddy MS, Vedamuthu ER and Reinbold GW (1971(b). Component balance in mixed lactic cultures when subjected to normal presentation procedures. J Dairy Sci.54:745.

Reddy MS. Vedamuthu ER, Washam CJ and Reinbold GW (1972(a). Agar medium for differential enumeration of lactic Streptococci. Appl. Microbiol. 24:947-952.

Reddy MS, Vedamuthu ER, Washam CJ and Reinbold GW (1972(b). Associative growth studies in three – strain mixtures of lactic Streptococci. Appl. Microbiol.25:953-957.

Reinbold GW, and Reddy MS (1974). Sensitivity or resistance of dairy starter and associated microorganisms to selected antibiotics. J Milk Food Technol. 37:517-521.

Reddy, MS, Reinbold GW, and Williams FD (1973). Inhibition of propionibacteria by antibiotic and antimicrobial agents. J Milk

Food Technol. 36:564-569.

Vecchi ED and Drago L (2006). Lactobacillus sporogenes or bacillus coagulans: misidentification or mislabeling? International Journal of Probiotics and Prebiotics1(1): 3-10.

Vorobjeva LI, Khodjaev EY and Vorobjeva NV (2008). Propionic acid bacteria as Probiotics. Microbial Ecology in Health and Disease20:109-112.

Warminiska-Radiko I, Laniewske-Moroz L and Babuchowski A (2006). Influence of Propionibacterium on selected pathogenic microorganisms. 3rd International symposium on Propioni-bacterium, Zurich. 8-11 July: 36.