Development and Application of HPLC Method for Clinical Pharmacokinetic Study of Domperidone

DOI:

https://doi.org/10.37285/ijpsn.2016.9.6.3

Authors

  • Prasad Neerati
  • Bhargavi Latha A
  • Y. Shravan Kumar
  • Y Madhusudan Rao

Abstract

A simple and sensitive high performance liquid chromatographic method for quantification of domperidone in human serum was developed and validated. Domperi-done and internal standard (IS) propranolol hydrochloride were extracted into acetonitrile and separated using an isocratic mobile phase on a Phenomenx C18 column. The eluent was monitored by UV detector at 270 nm at a flow rate of 1.0 mL min–1. The linearity range of proposed  method was 2.5–1000 ng/ml. The intra-day and inter-day coefficient of variation and percent error values of the assay method were less than 15% and mean recovery was more than 97 and 96% for domperidone and IS, respectively. The method was found to be precise, accurate and specific during the study. The method was successfully applied for pharmacokinetic study of domperidone in humans.

 

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Keywords:

HPLC, Eluent, Precise, Accurate, Domperidone

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Published

2016-11-30

How to Cite

1.
Neerati P, A BL, Kumar YS, Rao YM. Development and Application of HPLC Method for Clinical Pharmacokinetic Study of Domperidone . Scopus Indexed [Internet]. 2016 Nov. 30 [cited 2024 Dec. 11];9(6):3531-5. Available from: https://www.ijpsnonline.com/index.php/ijpsn/article/view/890

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Section

Research Articles

References

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