Spectroscopic Determination of Aspirin and Omeprazole by Absorbance Ratio and Multicomponent Mode Method

DOI:

https://doi.org/10.37285/ijpsn.2017.10.6.4

Authors

  • Hajera Khan
  • Swapna S Bandewar
  • Mohammad Zameeruddin
  • Vishvanath B Bharkad

Abstract

Here we describe a simple, rapid and accurate method for simultaneous assay of aspirin and omeprazole. The first method was Absorbance ratio method (Method 1) and second method was multi component mode method of analysis (Method 2).  Methanol: water (8:2) was used as solvent for both methods, using 293 nm as isobestic point for absorbance ratio method. The wavelength ranges 275.80 nm for aspirin and 302.20 nm for omeprazole for method 2, which represents the absorbance maxima of both drugs respectively. Beer’s law was applied in the concentration ranges of 2-14μg/mL and 2-18 μg/mL for aspirin and omeprazole, respectively, in absorbance ratio methods. The percentage assay was found to be in the range 99.74 to 100 % for aspirin and 99.69 to 99.9 % for omeprazole for both the methods. Recovery was found in the range of 99.74 –100.14 % for aspirin and omeprazole for both methods. The analysis data has been validated statistically and recovery studies confirmed the accuracy and reproducibility of the proposed methods, which were carried out according to the ICH guidelines.   

 

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Keywords:

Aspirin, Omeprazole, Absorbance ratio, Multicomponent; stability

Downloads

Published

2017-11-30

How to Cite

1.
Khan H, Bandewar SS, Zameeruddin M, Bharkad VB. Spectroscopic Determination of Aspirin and Omeprazole by Absorbance Ratio and Multicomponent Mode Method. Scopus Indexed [Internet]. 2017 Nov. 30 [cited 2024 Jun. 20];10(6):3900-5. Available from: https://www.ijpsnonline.com/index.php/ijpsn/article/view/871

Issue

Section

Research Articles

References

alidation of stability indicating HPTLC method for determination of Prasugrel, J. Chem. Pharm. Res 2: 907-913.
Chodvadiya FJ, Thula KC and Maheshwari DG (2015). Simultaneous Estimation of Aspirin and Lansoprazole by RP–HPLC Method. International Journal of Recent Scientific Research 6(4): 3385-3390.
Farid NA, McIntosh M, Garofolo F, Wong E, Shwajch A, Kennedy M, Young M, Sarkar P, Kawabata K, Takahashi M and Pang H (2007). Determination of the active and inactive metabolites of prasugrel in human plasma by liquid chromatography/tandem mass spectrometry. Rapid. Commun. Mass. Spectrom 21: 169-79.
Godavariya D, Prajapati B, P. Marolia and Shah SA (2012). Development and Validation of RP-HPLC Method for Simultaneous Estimation of Rosuvastatin Calcium & Aspirin in Marketed Formulation. International Research Journal of Pharmacy 3(8): 173-175.
ICH (2005). ICH-Guidelines Q2 (R1), Validation of Analytical Procedures: Text and Methodology, 1-13.
Indian pharmacopoeia (2010). 6thedition. Government of India, Ministry of Health and Family Welfare, Ghaziabad, New Delhi, The Indian pharmacopoeia commission publisher, 3: 1813-1814.
Jain D, Jain N and Verma J (2012). RP-HPLC Method for Simultaneous Estimation of Aspirin and Prasugrel in Binary Combination. International Journal of Pharmaceutical Sciences and Drug Research 4(3): 218-221.
Kamal A.H, Samah F. El-Malla and Hammad S.F (2016). A Review on Uv Spectrophotometric Methods For Simultaneous Multicomponent Analysis. European Journal of Pharmaceutical and Medical Research 3(2): 348-353.
Kumaraswamy G, Repeudi LK and Vijayaprakash K (2016).Validated Spectrophotometric Method For Simultaneous Estimation Of Rosuvastatin Calcium and Aspirin in Tablet Dosage Forms. International Journal of Pharmaceutical Chemistry and Analysis 3(2): 90-98.
Lukram O, Zarapkar M, Jha CK, Parmar S, Tomar KS and Hande A (2012). Electrospray ionization LC-MS/MS validated method for the determination of the active metabolite (R-138727) of prasugrel in human plasma and its application to a bioequivalence study. Drug. Test. Anal 4: 58-166.
Patel BB, Shah BB, Gohil KN and Patel PM (2012). Development and Validation of Spectrophotometric Method for Simultaneous Estimation of Rosuvastatin Calcium and Aspirin in Bulk and Pharmaceutical Dosage Form. International Journal of Research in Pharmacy and Science 2(2): 115-122.
Purkar AJ, Balap AR, Jadhav SB and Chaudhari PD (2012). Development and Validation of UV Spectrophotometric Method for Simultaneous Determination of Rosuvastatin Calcium and Aspirin in its Pure and Pharmaceutical Dosage Forms. International Journal of Pharmaceutical and Chemical Sciences 1(3): 1008-1012.
Rajput S and Fanse S (2015). RP-HPLC Method for Simultaneous Estimation of Lansoprazole and Aspirin in Bulk and Laboratory Mixture. Journal of Advanced Pharmacy Education & Research 5(2): 87-93.
Skoog DA, Hooler FJ and Timothy A (1998). Principles of Instrumental Analysis. 5th Edition, M. Sherman, J. Bortel, F. Messina (EDS), Stanford University. Saunders College Publication, London: 3.
Solanki C and Patel N (2012). Development and Validation of RP-HPLC Method for Simultaneous Estimation of Rosuvastatin Calcium and Aspirin in Capsule Dosage Form. International Journal of Pharma and Bio Sciences, 3(3): 577-585.
Wickremsinhe ER, Tian Ye, Ruterbories KJ, Verburg EM, Weerakkody GJ, Kurihara A and Farid NA (2007). Stereoselective metabolism of prasugrel in humans using a novel chiral liquid chromatographytandem mass spectrometry method. Drug Metab Dispos 35: 917-921.