Evaluation of Hemolytic and Anti-Hemolytic Activity of the Aerial Parts of Sonchus Oleraceus Extracts
DOI:
https://doi.org/10.37285/ijpsn.2017.10.3.7Abstract
Plants have been used as alternative remedy for the treatment of various ailments since ancient times. The present study aimed to evaluate some of the biological activities of the plant Sonchus oleraceus in vitro including the hemolytic and antihemolytic effect. Hydromethanolic 80% and aqueous extracts of the aerial parts of plant were prepared using ultrasound-assisted extraction. Phyto-chemical analysis, hemolytic and anti-hemolytic activity of both extracts of Sonchus oleraceus were assessed. Phyto-chemical screening revealed the presence of tannins, carbohydrates, flavonoids, saponins, and phenolic compounds in both extracts. Hemolytic activity assessment has been conducted, using spectrophotometric method. The extracts had low hemolytic effect towards human erythrocytes in concentration-dependent manner.The extracts showed moderate membrane stabilizing effect against hypotonic-induced hemolysis at concentration range of (250-1000 µg/ml). This study also showed that extracts had potential antioxidant activity through the inhibition of H2O2 induced hemolysis. Both extracts showed remarkable antihemolytic activity against H2O2 induced hemolysis at all tested concentrations. It was concluded that the extracts of S. oleraceus manifest low hemolytic effect, and had antihemolytic activity. However, these effects need to be confirmed using in vivo models.
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Keywords:
Sonchus Oleraceus, Hemolytic activity, Erythrocytes membrane, Membrane-stabilizing property, Antihemolytic, Antioxidant
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Published
2017-05-31
How to Cite
1.
Dima J, Raghda L, Jalil GA. Evaluation of Hemolytic and Anti-Hemolytic Activity of the Aerial Parts of Sonchus Oleraceus Extracts. Scopus Indexed [Internet]. 2017 May 31 [cited 2024 Apr. 26];10(3):3745-51. Available from: https://www.ijpsnonline.com/index.php/ijpsn/article/view/847
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Research Articles
References
Ahmad WS, Shah K.W, and Ashfaq MA (2012). Preliminary phytochemical investigation and thin layer chromatography of rheum emodi. Int Res J Pharm, 3: 176-177.
Albalawi MAD, Bashir NAO, and Tawfik A (2015). Anticancer and antifolate activities of extracts of six Saudi Arabian wild plants used in folk medicine. J Life Sci 9: 334-340.
Amini E, Nabiuni M, Baharara J, Parivar K, and Asili J (2014). Hemolytic and Cytotoxic Effects of SaponinLike Compounds Isolated From Persian Gulf Brittle Star (Ophiocomaerinaceus), J Coastal Life Med, 2: 762-768.
Ananthi T, and Chitra M, (2013). screening of in vitro anti-inflammatory activity of Michelia Champaca LINN. FLOWERS. Int J Pharm sci res, 4: 721-723.
Anbarasi A, and Vidhya R (2015). Evaluation of in vitro anti- inflammatory activity of tephrosiapurpurea (seed). Asian J. Pharm. Res, 5: 83-89.
Asif F, Boby AZ, Alam N, Taraquzzaman M, Chowdhury SR, and Rashid MA (2014). Canvassing of thrombolytic, cytotoxic, and erythrocyte membrane-stabilizing attributes in in vitro screening of Gynocardiaodorata. J Anal Sci Technol, 5: 2-7.
Attard E, and Cuschieri A (2009). In vitro immunomodulatory activity of various extracts of Maltese plants from the Asteraceae family. J Med Plants Res, 3: 457-461.
Awe EO, Makinde JM, Adeloye OA, and Banjoko SO (2009). Membrane Stabilizing Activity of Russelia Equisetiformis, Schlecht & Chan. J Nat Prod, 2: 3-9.
Borra SK, Gurumurthy P, Mahendra J, Jayamathi KM, Cherian CN, and Chand R (2013). Antioxidant and Free Radical Scavenging Activity of Curcumin Determined By Using Different in vitro and ex vivo Models. J Med Plants Res, 7: 2680-2690.
Chakraborty D, and Shah B (2011). Antimicrobial, Antioxidative and Antihemolytic Activity OF Piper Betel Leaf Extracts. Int J Pharm Pharm Sci, 3:192-199.
Colegate SM and Molyneux RJ (2008). Bioactive Natural Products: Detection, Isolation, and Structural Determination, 2nd ed. CRC Press Taylor & Francis Group, New York.
Dai J and Mumper RJ (2010). Plant Phenolics: Extraction, Analysis and their Antioxidant and Anticancer Properties. Mol, 15: 7313-7352.
Debnath PC, Das A, Islam A, Islam A, and Hassan M (2013). Membrane Stabilization e A Possible Mechanism of Action For The Anti-Inflammatory Activity of a Bangladeshi Medicinal Plant: Erioglossumrubiginosum (Bara Harina). Pharmacogn J, 5: 104-107.
Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Eslami B, and Rahmani ZP (2010). Antioxidant and Antihaemolytic Activities of the Leaves of Kefe cumin (Laser trilobum L) Umbelliferae. Trop J Pharm Res, 9: 441-449.
Freitas MVD, Netto RC, Huss JCD, Souza TM, Costa JO, Firmino CB, and Silva NP (2008). Influence of aqueous crude extracts of medicinal plants on the osmotic stability of human erythrocytes. Toxicol In Vitro, 22: 219-224.
Hossain MM, Ahamed SK, Dewan SMR, Hassan MM, Istiaq A, Islam MS, and Moghal MMR (2014). In vivo antipyretic, antiemetic, in vitro membrane stabilization, antimicrobial, and cytotoxic activities of different extracts from Spilanthe-spaniculata leaves. Biol Res, 47: 2-9.
Jimoh FO, Adedapo AA, and Afolayan AJ (2011). Comparison of the nutritive value, antioxidant and antibacterial activities of Sonchus asper and Sonchusoleraceus. Rec. Nat. Prod, 5: 29-42.
Joshan D.S, and Rawal S (2012). Comparative evaluation of antioxidant and antihemolytic capacities of plants of indian origin using multiple antioxidant assays. Int J Phytopharm, 2: 107-115.
Khalili M, Ebrahimzadeh MA, and Safdari Y (2014). Antihemolytic Activity of Thirty Herbal Extracts in Mouse Red Blood Cells. ArhHig Rada Toksikol, 65: 399-406.
Kuddus MR, Alam MS, Chowdhury SR, Rumi F, Sikder MD, and Rashid MA (2012). Evaluation of Membrane Stabilizing Activity, Total Phenolic Content, Brine Shrimp Lethality Bioassay, Thrombolytic and Antimicrobial Activities of TagetespatulaL. J PharmacognPhytochem, 1: 57-62.
Lakshmi G, Smitha N, Ammu SV, and Priya L, and Bhaskarakv (2014). Phytochemical profile, in vitro antioxidant and hemolytic activities of various leaf extract of Nymphaea nouchalilinn: an in vitro study. Int J Pharm Pharm Sci, 6: 548-552.
Lohith K, Vijay R, Pushpalatha KC, and Joshi CG (2013). In-vitro cytotoxic study of moullavaspicata (dalz.) nicolson leaf extract. Int Res J Pharm. App Sci 3: 38-42.
Mannan MM, Akhter R, Shahriar M, and Bhuiyan MA (2015). Evaluation of in vitro membrane stabilizing activity and thrombolytic activity of averrhoabilimbileaf extracts. Int J Pharm, 5: 357-360.
Manthey JA, Grohmann K, and Guthrie N (2001). Biological properties of citrus flavonoids pertaining to cancer and inflammation. Curr Med Chem, 8: 135-53.
Muthu S, and Durairaj B (2015). Inhibitory effect of hydroethanolic extracts Of Annona Muricataon human platelet aggregation and hemolysis in vitro. Int J Pharm Pharm Res (IJPPR,) 2: 207-214.
Noudeh GD, Sharififar F, Khatib M, Behravan E, and Afzadi M.A (2010). Study of Aqueous Extract of Three Medicinal Plants on Cell Membrane–Permeabilizing and Their Surface Properties. Afr J Biotechnol, 9: 110-116.
OliveiraV, Carneiro A, Cauper G, and Pohlit AMP (2009). In Vitro Screening of Amazonian Plants For Hemolytic Activity and Inhibition of Platelet Aggregation in Human Blood. Acta Amazonica, 39: 973-980.
Oluboade OO, Morakinyo MA, Monijesu IJ, Olasunmbo AE, Feyisayo AK, and Ayinke AB (2015). Biological Activities (Anti-Inflammatory and Anti-oxidant) of Fractions and Methanolic Extract of PhilonotisHastata(DubyWijk&Margadant). Afr J Tradit Complement Altern Med, 12: 50-55.
Pandey A, and Tripathi S (2014). Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. J Pharmacogn Phytochem, 2: 115-119.
Patel D, and Desai S (2016). Phytochemical screening, in vitro anti-microbial and anti-inflammatory activity of methanolic extract of Aster lanceolatuswilld leaves. Int J Med Res, 1: 26-30.
Rajagopal PL, Premaletha K, and Sreejith KR (2016). Anti-inflammatory screening on the flowers of trichodesmaindicumlinn. World Wide J Multidiscip Res Dev, 2: 19-21.
Rajalakshmi GR, and Harindran J (2013). Anti-Inflammatory Activity of SamaderaindicaLeaves by Membrane Stabilization. Int J Pharm sci res, 4: 721-723.
Rao CS (2015). Study of Pharmacological Anti-inflammatory Activity of PsidiumguajavaLinn. Leaves and Bark by Tannin Fractions. J Pharmacogn Phytochem, 3: 37-45.
Sangeetha G, and Vidhya R, (2016). In Vitro Anti-Inflammatory Activity of Different Parts of Pedalium murex (L.). Int J Herbal Med, 4: 31-36.
Saradha M, Paulsamy S, and Vinitha R (2013). Antioxidant and Antihemolytic Activity of an Endangered Plant Species, hildegardiapopulifolia(roxb.) schott & endl. Asian J Pharm clin res, 6: 135-137.
Saxena M, Saxena J, Nema R, Singh D, and Gupta A (2013). Phytochemistry of medicinal plants. J Pharm Phytochem, 1: 168-183.
Shah S, Khan K, Shah S, Chishti K, Pirzada S, Khan M, and Farid A (2011). Evaluation of Phytochemicals and Antimicrobial Activity of White and Blue Capitulum and Whole Plant of Silybum Marianum. World App Sci J, 12: 1139-1144.
ShaileshG, SeemaK, and DwivediS (2011). In-Vitro anti-inflammatory activity of Sarcostemmaacidum Wight. &Arn. Indian herb by Human red blood cell membrane stabilization method. Int J Pharm Teaching & Practices, 2: 184-188.
Shirwaikar A, Devi S and Siju EN (2011). Anti-Inflammatory Activity of Thespesiapopulnea fruits by Membrane Stabilization. Int J Pharm Tech Res, 3: 2060-2063.
Shobana S, and Vidhya R (2016). Evaluation of In vitro Hemolytic Activity of Different Parts of Abutilon Indicum (Linn.). World J Pharm Pharm Sci, 5: 1182-1196.
Shwetha RJ, Tahareen S, and Myrene RD (2016). Antioxidant and anti-inflammatory activity of tinosporacordifoliausing in vitro models. J Chem Biol Phys Sci, 6: 497-512.
Singh GK, and Jain SK (2014). Preliminary phytochemical sereening and in vitro antioxidant activity of extracts of whole plant of Sonchusoleraceusasteraceae. Res J Pharm Sci 3: 1-12.
Sultana B, Anwar F, and Ashraf M (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Mol, 14: 2167-2180.
Sumathi S, and Anuradha R (2016). In vitro anti-inflammatory acitivty of flower extract of couroupitaguianensisaubl. Innovare J Ayurvedic Sci, 4: 16-18.
Ukwuani AN, and Ahmad HB (2015). In vitro anti-inflammatory activity of parkiabiglobosa fruit bark extract. Int J Life-SciSci Res (IJLSSR), 1: 8-11.
UkwuaniAN, and Hassan IB (2015). In vitro anti-inflammatory activity of hydromethanolic seed, fruit and leave extracts of capsicum chinense(red pepper). Eur J Biomed Pharm sci, 2: 57-65.
Urbańska N, Nartowska J, Skorupska A, Ruszkowski D, Giebultowicz J, and Olszowska O (2009). Determination and Haemolytic Activity of Saponins in Hairy Root Culture of Platycodongrandiforum A.DC.herbapolonica, 55: 103-108.
Vidya V, Somayaji TY, Pooja S, PATIL S, Fernandes R, and Krishna AB (2015). Assessment of Membrane Stabilization, Antioxidant and Thrombolytic Potential of Lutein- an in-vitro Study. Int J Pharm Sci Res, 6: 4478-4483.
Yuliana P, Laconi EB, Wina E, and Jayanegara A (2014). extraction of tannins and saponins from plant sources and their effects on in vitro methanogenesis and rumen fermentation. J Indonesian Trop Anim Agric, 39: 91-97.
Žlabur JS, Voća S, Dobričević N, Brnčić M, Dujmić F, and Brnčić SR (2015). Optimization of ultrasound assisted extraction of functional ingredients from Stevia rebaudiana Bertoni leaves. Int. Agrophys, 29: 23-237.
Zohra M, and Fawzia A (2014). Hemolytic activity of different herbal extracts used in algeria. Int J Pharm Sci Res (IJPSR), 5: 495-500.
Albalawi MAD, Bashir NAO, and Tawfik A (2015). Anticancer and antifolate activities of extracts of six Saudi Arabian wild plants used in folk medicine. J Life Sci 9: 334-340.
Amini E, Nabiuni M, Baharara J, Parivar K, and Asili J (2014). Hemolytic and Cytotoxic Effects of SaponinLike Compounds Isolated From Persian Gulf Brittle Star (Ophiocomaerinaceus), J Coastal Life Med, 2: 762-768.
Ananthi T, and Chitra M, (2013). screening of in vitro anti-inflammatory activity of Michelia Champaca LINN. FLOWERS. Int J Pharm sci res, 4: 721-723.
Anbarasi A, and Vidhya R (2015). Evaluation of in vitro anti- inflammatory activity of tephrosiapurpurea (seed). Asian J. Pharm. Res, 5: 83-89.
Asif F, Boby AZ, Alam N, Taraquzzaman M, Chowdhury SR, and Rashid MA (2014). Canvassing of thrombolytic, cytotoxic, and erythrocyte membrane-stabilizing attributes in in vitro screening of Gynocardiaodorata. J Anal Sci Technol, 5: 2-7.
Attard E, and Cuschieri A (2009). In vitro immunomodulatory activity of various extracts of Maltese plants from the Asteraceae family. J Med Plants Res, 3: 457-461.
Awe EO, Makinde JM, Adeloye OA, and Banjoko SO (2009). Membrane Stabilizing Activity of Russelia Equisetiformis, Schlecht & Chan. J Nat Prod, 2: 3-9.
Borra SK, Gurumurthy P, Mahendra J, Jayamathi KM, Cherian CN, and Chand R (2013). Antioxidant and Free Radical Scavenging Activity of Curcumin Determined By Using Different in vitro and ex vivo Models. J Med Plants Res, 7: 2680-2690.
Chakraborty D, and Shah B (2011). Antimicrobial, Antioxidative and Antihemolytic Activity OF Piper Betel Leaf Extracts. Int J Pharm Pharm Sci, 3:192-199.
Colegate SM and Molyneux RJ (2008). Bioactive Natural Products: Detection, Isolation, and Structural Determination, 2nd ed. CRC Press Taylor & Francis Group, New York.
Dai J and Mumper RJ (2010). Plant Phenolics: Extraction, Analysis and their Antioxidant and Anticancer Properties. Mol, 15: 7313-7352.
Debnath PC, Das A, Islam A, Islam A, and Hassan M (2013). Membrane Stabilization e A Possible Mechanism of Action For The Anti-Inflammatory Activity of a Bangladeshi Medicinal Plant: Erioglossumrubiginosum (Bara Harina). Pharmacogn J, 5: 104-107.
Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Eslami B, and Rahmani ZP (2010). Antioxidant and Antihaemolytic Activities of the Leaves of Kefe cumin (Laser trilobum L) Umbelliferae. Trop J Pharm Res, 9: 441-449.
Freitas MVD, Netto RC, Huss JCD, Souza TM, Costa JO, Firmino CB, and Silva NP (2008). Influence of aqueous crude extracts of medicinal plants on the osmotic stability of human erythrocytes. Toxicol In Vitro, 22: 219-224.
Hossain MM, Ahamed SK, Dewan SMR, Hassan MM, Istiaq A, Islam MS, and Moghal MMR (2014). In vivo antipyretic, antiemetic, in vitro membrane stabilization, antimicrobial, and cytotoxic activities of different extracts from Spilanthe-spaniculata leaves. Biol Res, 47: 2-9.
Jimoh FO, Adedapo AA, and Afolayan AJ (2011). Comparison of the nutritive value, antioxidant and antibacterial activities of Sonchus asper and Sonchusoleraceus. Rec. Nat. Prod, 5: 29-42.
Joshan D.S, and Rawal S (2012). Comparative evaluation of antioxidant and antihemolytic capacities of plants of indian origin using multiple antioxidant assays. Int J Phytopharm, 2: 107-115.
Khalili M, Ebrahimzadeh MA, and Safdari Y (2014). Antihemolytic Activity of Thirty Herbal Extracts in Mouse Red Blood Cells. ArhHig Rada Toksikol, 65: 399-406.
Kuddus MR, Alam MS, Chowdhury SR, Rumi F, Sikder MD, and Rashid MA (2012). Evaluation of Membrane Stabilizing Activity, Total Phenolic Content, Brine Shrimp Lethality Bioassay, Thrombolytic and Antimicrobial Activities of TagetespatulaL. J PharmacognPhytochem, 1: 57-62.
Lakshmi G, Smitha N, Ammu SV, and Priya L, and Bhaskarakv (2014). Phytochemical profile, in vitro antioxidant and hemolytic activities of various leaf extract of Nymphaea nouchalilinn: an in vitro study. Int J Pharm Pharm Sci, 6: 548-552.
Lohith K, Vijay R, Pushpalatha KC, and Joshi CG (2013). In-vitro cytotoxic study of moullavaspicata (dalz.) nicolson leaf extract. Int Res J Pharm. App Sci 3: 38-42.
Mannan MM, Akhter R, Shahriar M, and Bhuiyan MA (2015). Evaluation of in vitro membrane stabilizing activity and thrombolytic activity of averrhoabilimbileaf extracts. Int J Pharm, 5: 357-360.
Manthey JA, Grohmann K, and Guthrie N (2001). Biological properties of citrus flavonoids pertaining to cancer and inflammation. Curr Med Chem, 8: 135-53.
Muthu S, and Durairaj B (2015). Inhibitory effect of hydroethanolic extracts Of Annona Muricataon human platelet aggregation and hemolysis in vitro. Int J Pharm Pharm Res (IJPPR,) 2: 207-214.
Noudeh GD, Sharififar F, Khatib M, Behravan E, and Afzadi M.A (2010). Study of Aqueous Extract of Three Medicinal Plants on Cell Membrane–Permeabilizing and Their Surface Properties. Afr J Biotechnol, 9: 110-116.
OliveiraV, Carneiro A, Cauper G, and Pohlit AMP (2009). In Vitro Screening of Amazonian Plants For Hemolytic Activity and Inhibition of Platelet Aggregation in Human Blood. Acta Amazonica, 39: 973-980.
Oluboade OO, Morakinyo MA, Monijesu IJ, Olasunmbo AE, Feyisayo AK, and Ayinke AB (2015). Biological Activities (Anti-Inflammatory and Anti-oxidant) of Fractions and Methanolic Extract of PhilonotisHastata(DubyWijk&Margadant). Afr J Tradit Complement Altern Med, 12: 50-55.
Pandey A, and Tripathi S (2014). Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. J Pharmacogn Phytochem, 2: 115-119.
Patel D, and Desai S (2016). Phytochemical screening, in vitro anti-microbial and anti-inflammatory activity of methanolic extract of Aster lanceolatuswilld leaves. Int J Med Res, 1: 26-30.
Rajagopal PL, Premaletha K, and Sreejith KR (2016). Anti-inflammatory screening on the flowers of trichodesmaindicumlinn. World Wide J Multidiscip Res Dev, 2: 19-21.
Rajalakshmi GR, and Harindran J (2013). Anti-Inflammatory Activity of SamaderaindicaLeaves by Membrane Stabilization. Int J Pharm sci res, 4: 721-723.
Rao CS (2015). Study of Pharmacological Anti-inflammatory Activity of PsidiumguajavaLinn. Leaves and Bark by Tannin Fractions. J Pharmacogn Phytochem, 3: 37-45.
Sangeetha G, and Vidhya R, (2016). In Vitro Anti-Inflammatory Activity of Different Parts of Pedalium murex (L.). Int J Herbal Med, 4: 31-36.
Saradha M, Paulsamy S, and Vinitha R (2013). Antioxidant and Antihemolytic Activity of an Endangered Plant Species, hildegardiapopulifolia(roxb.) schott & endl. Asian J Pharm clin res, 6: 135-137.
Saxena M, Saxena J, Nema R, Singh D, and Gupta A (2013). Phytochemistry of medicinal plants. J Pharm Phytochem, 1: 168-183.
Shah S, Khan K, Shah S, Chishti K, Pirzada S, Khan M, and Farid A (2011). Evaluation of Phytochemicals and Antimicrobial Activity of White and Blue Capitulum and Whole Plant of Silybum Marianum. World App Sci J, 12: 1139-1144.
ShaileshG, SeemaK, and DwivediS (2011). In-Vitro anti-inflammatory activity of Sarcostemmaacidum Wight. &Arn. Indian herb by Human red blood cell membrane stabilization method. Int J Pharm Teaching & Practices, 2: 184-188.
Shirwaikar A, Devi S and Siju EN (2011). Anti-Inflammatory Activity of Thespesiapopulnea fruits by Membrane Stabilization. Int J Pharm Tech Res, 3: 2060-2063.
Shobana S, and Vidhya R (2016). Evaluation of In vitro Hemolytic Activity of Different Parts of Abutilon Indicum (Linn.). World J Pharm Pharm Sci, 5: 1182-1196.
Shwetha RJ, Tahareen S, and Myrene RD (2016). Antioxidant and anti-inflammatory activity of tinosporacordifoliausing in vitro models. J Chem Biol Phys Sci, 6: 497-512.
Singh GK, and Jain SK (2014). Preliminary phytochemical sereening and in vitro antioxidant activity of extracts of whole plant of Sonchusoleraceusasteraceae. Res J Pharm Sci 3: 1-12.
Sultana B, Anwar F, and Ashraf M (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Mol, 14: 2167-2180.
Sumathi S, and Anuradha R (2016). In vitro anti-inflammatory acitivty of flower extract of couroupitaguianensisaubl. Innovare J Ayurvedic Sci, 4: 16-18.
Ukwuani AN, and Ahmad HB (2015). In vitro anti-inflammatory activity of parkiabiglobosa fruit bark extract. Int J Life-SciSci Res (IJLSSR), 1: 8-11.
UkwuaniAN, and Hassan IB (2015). In vitro anti-inflammatory activity of hydromethanolic seed, fruit and leave extracts of capsicum chinense(red pepper). Eur J Biomed Pharm sci, 2: 57-65.
Urbańska N, Nartowska J, Skorupska A, Ruszkowski D, Giebultowicz J, and Olszowska O (2009). Determination and Haemolytic Activity of Saponins in Hairy Root Culture of Platycodongrandiforum A.DC.herbapolonica, 55: 103-108.
Vidya V, Somayaji TY, Pooja S, PATIL S, Fernandes R, and Krishna AB (2015). Assessment of Membrane Stabilization, Antioxidant and Thrombolytic Potential of Lutein- an in-vitro Study. Int J Pharm Sci Res, 6: 4478-4483.
Yuliana P, Laconi EB, Wina E, and Jayanegara A (2014). extraction of tannins and saponins from plant sources and their effects on in vitro methanogenesis and rumen fermentation. J Indonesian Trop Anim Agric, 39: 91-97.
Žlabur JS, Voća S, Dobričević N, Brnčić M, Dujmić F, and Brnčić SR (2015). Optimization of ultrasound assisted extraction of functional ingredients from Stevia rebaudiana Bertoni leaves. Int. Agrophys, 29: 23-237.
Zohra M, and Fawzia A (2014). Hemolytic activity of different herbal extracts used in algeria. Int J Pharm Sci Res (IJPSR), 5: 495-500.
