Evaluation of Antiurolithiatic Activity of Lawsonia inermis Linn. in Rats
Corresponding Author(s) : Kruti M Patel
krutspatel90004@gmail.com
International Journal of Pharmaceutical Sciences and Nanotechnology,
Vol 10 No 3 (2017): May-June 2017
Abstract
This study was conducted to evaluate the potential antiurolithiatic effects of Lawsonia Inermis Linn. in rat models. Animals were divided into seven groups and urolithiasis was induced by ethylene glycol (0.75% v/v) in drinking water to all groups (Groups II-VII) except normal control (Group I) for 28 days. Methanolic extracts of Lawsonia inermis (MELI) bark (300 & 500 mg/kg, p.o.) were administered once daily, from 15th day to 28th day as curative regimen and from 1st day to 28th day as preventive regimen. Cystone (750 mg/kg, p.o.) was used as a standard drug for comparison. After 28 days, various biochemical parameters like urine volume, pH were measured. Calcium, phosphate and oxalate were measured in urine and kidney homogenate. Serum creatinine, uric acid and urea nitrogen were estimated. Histopathology of kidney was investigated. Treatment with the MELI extract significantly (p<0.05) restored all elevated parameters including calcium, phosphate and oxalate in urine and kidney homogenate; creatinine, uric acid and urea nitrogen in serum when compared to model control group. The histopathological study of the kidney also supported the above results. It was concluded that methanolic extract of bark of Lawsonia inermis Linn. has significant antiurolithiatic effect in experimental rats.
Keywords
Lawsonia inermis Linn
Ethylene glycol
Urolithiasis
Cystone
PatelK. M., & K ShahS. (2017). Evaluation of Antiurolithiatic Activity of Lawsonia inermis Linn. in Rats. International Journal of Pharmaceutical Sciences and Nanotechnology, 10(3), 3728-3735. https://doi.org/10.37285/ijpsn.2017.10.3.5
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References
References
Agarwal K, and Varma R (2014). Ocimum gratissimum L.: A medical plant with promising antiurolithiatic activity. International Journal of Pharmaceutical Sciences and Drug Research 6: 78.
Agarwal P, Alok S, and Verma A (2014). An update on ayurvedic herb Heena (Lawsonia inermis): A review. International Journal of Pharmaceutical Sciences and Research 5: 330.
Aggarwal KP, Narula S, Kakkar M, and Tendon C (2013). Nephrolithiasis: Molecular mechanism of renal stone formation and the critical role played by modulators. Bio Med Research International 1.
Alok S, Jain SK, Verma A, Kumar M, and Sabharwal M (2013). Pathophysiology of kidney, gallbladder and urinary stones treatment with herbal and allopathic medicine: A review. Asian Pac J Trop Dis 3: 496.
Ashok P, Koti B, and Vishwanathswamy AHM (2010). Antiurolithiatic and antioxidant activity of Mimusops elengi on ethylene glycol induced urolithiasis in rats. Indian Journal of Pharmacology 46: 380.
Atodariya U, Barad R, Upadhyay S, and Upadhyay U (2013). Journal of Pharmacognosy and Phytochemistry Anti-Urolithiatic Activity of Dolichos Biflorus Seeds. Journal of Pharmacognosy and Phytochemistry 2: 209.
Baheti DG, and Kadam SS (2013). Antiurolithiatic activity of some traditional medicinal plants against calcium oxalate induced urolithiasis in rats. International Journal of Pharmaceutical, Chemical and Biological Sciences 3: 1276.
Chinnala HM, Shanigarm S, and Elsani MM (2013). Antiurolithiatic activity of the plant extracts of Solanum virginianum on ethylene glycol induced urolithiasis in rats. International Journal of Pharmacy and Biological Sciences 3: 328.
Dharmalingam SR, Madhappan R, Chidambaram K, Ramamurthy S, Gopal K, Swetha P, and Senthil KL (2014). Anti-urolithiatic activity of Melia azedarach Linn leaf extract in ethylene glycol-induced urolithiasis in male albino rats. Tropical Journal of Pharmaceutical Research 13: 391.
Divakar K, Pawar AT, Chandrasekhar SB, Dighe SB, and Divakar G (2010). Protective effect of the hydro-alcoholic extract of Rubia cordifolia roots against ethylene glycol induced urolithiasis in rats. Food and Chemical Toxicology 48: 1013.
Dixit P, koti BC, and Vishwanathswamy AHM (2014). Antiurolithiatic activity of Crashcal on ethylene glycol induced urolithiasis in rats. J Pharm Sci 4: 30.
Galani VJ, and Panchal RR (2014a). Antiurolithiatic activity of Centratherum anthelminticum (l.) Kuntze seeds against ethylene glycol induced urolithiasis inrats. International Journal of Phytotherapy Research 4: 29.
Galani VJ, and Panchal RR (2014b). In vitro evaluation of Centratherum anthelminticum seeds for antinephrolithiatic activity. J Homeop Ayurv Med 3: 1.
Gupta SK, Baghel MS, Bhuyan C, Ravishnhar B, Ashok BD, and Patil PD (2012). Evaluation of anti-urolithiatic activity of Pashanabhedadi Ghrita against experimentally induced renal calculi in rats. Avicema Journal of Phytomedicine 33: 429.
Hajzadeh MR, Rajaei Z, Sadeghian MH4, Hashemi N, and Keshavarzi Z (2011). Preventive effect of Cynodon dactylon against ethylene glycol-induced nephrolithiasis in male rats. Avicenna Journal of Phytomedicine 1: 14.
Hegde G, and Bhat P (2014). A review on the concept of urolithiasis in ayurveda.” An International Peer Reviewed Ayurved Journal 2: 1.
Hodgkinson A (1970). Determination of oxalic acid in biological material. Clin. Chem. 16: 547.
Joy JM, Prathyusha S, Mohanalakshmi S, Praveen Kumar AVS, and Kumar CKA (2012). Potent herbal wealth with litholytic activity: A review. International Journal of Innovative Drug Discovery 2: 66.
Karadi RV, Gadge N, Alagawadi KR, and Savadi RV (2006). Effect of Moringa oleifera Lam. root-wood on ethylene glycol induced urolithiasis in rats. J. Ethnopharmacol 105: 306.
Khaling M, Kumar S, and Vandana (2014). Current scenario of urolithiasis and the use of medicinal plants as antiurolithiatic agents in Manipur (North East India): A Review. International Journal of Herbal Medicine 2: 1.
Khan MA, and Pradhan D (2012). Antiurolithc Activity of Ceropegia bulbosa Extract in Rats. Der Pharmacia Sinica 3: 148.
Kirtikar KR and Basu BD (2005). Indian Medicinasl Plants. International book distributors, Dehradun.
Kokate CK, Purohit AP and Gokhale SB (1990). Pharmacognosy. Nirali Prakashan, Pune.
Manjula K, Rajendran K, Eevera T, and S Kumaran (2012). Effect of Costus igneus stem extract on calcium oxalate urolithiasis in albino rats. Urol Res 4: 499.
Nagal A, and Singla RK (2013). Herbal Resources with Antiurolithiatic Effects: A Review. Indo Global Journal of Pharmaceutical Sciences 3: 6.
Narayan MR, and Panda PK (2014). A conceptual patho-clinical study on the disorder of mutrabaha srotas w. S. R. To mutrashmari (urolithiasis). Int J Ayur Pharma Research 2: 30.
Nesa L, Munira S, Mollika S, Islam MM, Choin H, Chouduri AU, and Naher N (2014). Evaluation of analgesic, anti-inflammatory and CNS depressant activities of methanolic extract of Lawsonia inermis barks in mice. Avicenna Journal of Phytomedicine 4: 287.
Padmalochana K, and Dhana Rajan KS (2015). In-vivo nephroprotective potential from leaves extracts of Andrographis paniculata. World Journal of Pharmacy and Pharmaceutical Sciences 4: 1426.
Pareta SK, Patra KC, Mazumder PM, and Sasmal D (2011). Establishing the Principal of Herbal Therapy for Antiurolithiatic Activity: A Review. Journal of Pharmacology and Toxicology 6: 321.
Semwal RB, Semwal DK, Combrinck S, Jones CC, and Viljoen A (2014). Lawsonia inermis L. (henna): Ethnobotanical, phytochemical and pharmacological aspects. Journal of Ethnopharmacology 155: 80.
Shelke TT, Bhaskar VH, Gunjegaokar SM, Antre RV, and Jha U (2014). A pharmacological appraisal of medicinal plants with antilithiatic activity. World Journal of Pharmacy and Pharmaceutical Sciences 3: 447.
Shukla AB, Mandavia DR, Barvaliya MJ, Baxi SN, and Tripathi CR (2014). Evaluation of anti-urolithiatic effect of aqueous extract of Bryophyllum pinnatum (Lam.) leaves using ethylene glycol-induced renal calculi. Avicema Journal of Phytomedicine 4: 151.
Singh M, Kaur M, Dangi CBS, and Singh H (2014). Phytochemical & TLC profile of Lawsonia inermis (Heena). International Journal for Pharmaceutical Research Scholars 3: 624.
Yadav RD, Alok S, Jain SK, Verma A, Mahor A, Bharti JP, and Jaiswal M (2011). Herbal plants used in the treatment of urolithiasis: A Review. International Journal of Pharmaceutical Sciences and Research 2: 1412.
Agarwal P, Alok S, and Verma A (2014). An update on ayurvedic herb Heena (Lawsonia inermis): A review. International Journal of Pharmaceutical Sciences and Research 5: 330.
Aggarwal KP, Narula S, Kakkar M, and Tendon C (2013). Nephrolithiasis: Molecular mechanism of renal stone formation and the critical role played by modulators. Bio Med Research International 1.
Alok S, Jain SK, Verma A, Kumar M, and Sabharwal M (2013). Pathophysiology of kidney, gallbladder and urinary stones treatment with herbal and allopathic medicine: A review. Asian Pac J Trop Dis 3: 496.
Ashok P, Koti B, and Vishwanathswamy AHM (2010). Antiurolithiatic and antioxidant activity of Mimusops elengi on ethylene glycol induced urolithiasis in rats. Indian Journal of Pharmacology 46: 380.
Atodariya U, Barad R, Upadhyay S, and Upadhyay U (2013). Journal of Pharmacognosy and Phytochemistry Anti-Urolithiatic Activity of Dolichos Biflorus Seeds. Journal of Pharmacognosy and Phytochemistry 2: 209.
Baheti DG, and Kadam SS (2013). Antiurolithiatic activity of some traditional medicinal plants against calcium oxalate induced urolithiasis in rats. International Journal of Pharmaceutical, Chemical and Biological Sciences 3: 1276.
Chinnala HM, Shanigarm S, and Elsani MM (2013). Antiurolithiatic activity of the plant extracts of Solanum virginianum on ethylene glycol induced urolithiasis in rats. International Journal of Pharmacy and Biological Sciences 3: 328.
Dharmalingam SR, Madhappan R, Chidambaram K, Ramamurthy S, Gopal K, Swetha P, and Senthil KL (2014). Anti-urolithiatic activity of Melia azedarach Linn leaf extract in ethylene glycol-induced urolithiasis in male albino rats. Tropical Journal of Pharmaceutical Research 13: 391.
Divakar K, Pawar AT, Chandrasekhar SB, Dighe SB, and Divakar G (2010). Protective effect of the hydro-alcoholic extract of Rubia cordifolia roots against ethylene glycol induced urolithiasis in rats. Food and Chemical Toxicology 48: 1013.
Dixit P, koti BC, and Vishwanathswamy AHM (2014). Antiurolithiatic activity of Crashcal on ethylene glycol induced urolithiasis in rats. J Pharm Sci 4: 30.
Galani VJ, and Panchal RR (2014a). Antiurolithiatic activity of Centratherum anthelminticum (l.) Kuntze seeds against ethylene glycol induced urolithiasis inrats. International Journal of Phytotherapy Research 4: 29.
Galani VJ, and Panchal RR (2014b). In vitro evaluation of Centratherum anthelminticum seeds for antinephrolithiatic activity. J Homeop Ayurv Med 3: 1.
Gupta SK, Baghel MS, Bhuyan C, Ravishnhar B, Ashok BD, and Patil PD (2012). Evaluation of anti-urolithiatic activity of Pashanabhedadi Ghrita against experimentally induced renal calculi in rats. Avicema Journal of Phytomedicine 33: 429.
Hajzadeh MR, Rajaei Z, Sadeghian MH4, Hashemi N, and Keshavarzi Z (2011). Preventive effect of Cynodon dactylon against ethylene glycol-induced nephrolithiasis in male rats. Avicenna Journal of Phytomedicine 1: 14.
Hegde G, and Bhat P (2014). A review on the concept of urolithiasis in ayurveda.” An International Peer Reviewed Ayurved Journal 2: 1.
Hodgkinson A (1970). Determination of oxalic acid in biological material. Clin. Chem. 16: 547.
Joy JM, Prathyusha S, Mohanalakshmi S, Praveen Kumar AVS, and Kumar CKA (2012). Potent herbal wealth with litholytic activity: A review. International Journal of Innovative Drug Discovery 2: 66.
Karadi RV, Gadge N, Alagawadi KR, and Savadi RV (2006). Effect of Moringa oleifera Lam. root-wood on ethylene glycol induced urolithiasis in rats. J. Ethnopharmacol 105: 306.
Khaling M, Kumar S, and Vandana (2014). Current scenario of urolithiasis and the use of medicinal plants as antiurolithiatic agents in Manipur (North East India): A Review. International Journal of Herbal Medicine 2: 1.
Khan MA, and Pradhan D (2012). Antiurolithc Activity of Ceropegia bulbosa Extract in Rats. Der Pharmacia Sinica 3: 148.
Kirtikar KR and Basu BD (2005). Indian Medicinasl Plants. International book distributors, Dehradun.
Kokate CK, Purohit AP and Gokhale SB (1990). Pharmacognosy. Nirali Prakashan, Pune.
Manjula K, Rajendran K, Eevera T, and S Kumaran (2012). Effect of Costus igneus stem extract on calcium oxalate urolithiasis in albino rats. Urol Res 4: 499.
Nagal A, and Singla RK (2013). Herbal Resources with Antiurolithiatic Effects: A Review. Indo Global Journal of Pharmaceutical Sciences 3: 6.
Narayan MR, and Panda PK (2014). A conceptual patho-clinical study on the disorder of mutrabaha srotas w. S. R. To mutrashmari (urolithiasis). Int J Ayur Pharma Research 2: 30.
Nesa L, Munira S, Mollika S, Islam MM, Choin H, Chouduri AU, and Naher N (2014). Evaluation of analgesic, anti-inflammatory and CNS depressant activities of methanolic extract of Lawsonia inermis barks in mice. Avicenna Journal of Phytomedicine 4: 287.
Padmalochana K, and Dhana Rajan KS (2015). In-vivo nephroprotective potential from leaves extracts of Andrographis paniculata. World Journal of Pharmacy and Pharmaceutical Sciences 4: 1426.
Pareta SK, Patra KC, Mazumder PM, and Sasmal D (2011). Establishing the Principal of Herbal Therapy for Antiurolithiatic Activity: A Review. Journal of Pharmacology and Toxicology 6: 321.
Semwal RB, Semwal DK, Combrinck S, Jones CC, and Viljoen A (2014). Lawsonia inermis L. (henna): Ethnobotanical, phytochemical and pharmacological aspects. Journal of Ethnopharmacology 155: 80.
Shelke TT, Bhaskar VH, Gunjegaokar SM, Antre RV, and Jha U (2014). A pharmacological appraisal of medicinal plants with antilithiatic activity. World Journal of Pharmacy and Pharmaceutical Sciences 3: 447.
Shukla AB, Mandavia DR, Barvaliya MJ, Baxi SN, and Tripathi CR (2014). Evaluation of anti-urolithiatic effect of aqueous extract of Bryophyllum pinnatum (Lam.) leaves using ethylene glycol-induced renal calculi. Avicema Journal of Phytomedicine 4: 151.
Singh M, Kaur M, Dangi CBS, and Singh H (2014). Phytochemical & TLC profile of Lawsonia inermis (Heena). International Journal for Pharmaceutical Research Scholars 3: 624.
Yadav RD, Alok S, Jain SK, Verma A, Mahor A, Bharti JP, and Jaiswal M (2011). Herbal plants used in the treatment of urolithiasis: A Review. International Journal of Pharmaceutical Sciences and Research 2: 1412.
