Development and Validation of Derivative Spectroscopic Method for Simultaneous Estimation of Cefadroxil and Probenecid

DOI:

https://doi.org/10.37285/ijpsn.2014.7.1.6

Authors

  • Pratik S Mehta
  • Pratik R. Patel
  • Rajesh R Parmar
  • M M K Modasiya
  • Dushyant A Shah

Abstract

A novel, simple, accurate, sensitive, precise and economical derivative spectroscopic method was developed and validated for the determination of cefadroxil and probenecid in synthetic mixture. First order derivative spectroscopy method was adopted to eliminate spectral interference. The method obeys Beer’s Law in concentration ranges of 4-36 μg/ml for cefadroxil and of 5-25 μg/ml of probenecid. The zero crossing point for cefadroxil and probenecid was 260 nm and 237.8 nm respectively in 0.1N HCl. The method was validated in terms of accuracy, precision, linearity, limits of detection, limits of quantitation. This method has been successively applied to synthetic mixture and no interference from the synthetic mixture’s excipients was found. 

 

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Keywords:

Cefadroxil, Probenecid, UV spectroscopic method, first order derivative spectroscopy, Method validation

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Published

2014-02-28

How to Cite

1.
Mehta PS, Patel PR, Parmar RR, Modasiya MMK, Shah DA. Development and Validation of Derivative Spectroscopic Method for Simultaneous Estimation of Cefadroxil and Probenecid. Scopus Indexed [Internet]. 2014 Feb. 28 [cited 2024 Dec. 10];7(1):2350-5. Available from: https://www.ijpsnonline.com/index.php/ijpsn/article/view/699

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Section

Research Articles

References

AnjumA (2011). Development and validation of new analytical methods for the estimation of cefadroxil in bulk and pharmaceutical dosage form. M.Pharm Thesis. Rajiv gandhi university.

Chetan P, Kamlesh P, Sen DJ, Badmanaban R and Ashish P (2010). Development and validation of spectrophotometric methods for the estimation of cefadroxil in tablet dosage forms. J Chem Pharm Res. 2(2): 163-167.

Chudhari SV, Karnik A, Adhikary A, Tandale RS and Vavia PR (2006). Simultaneous UV spectrophotometric method for estimation of cefuroxime axetil and probenecid from solid dosage form.Ind J Pharm Sci. 8(1): 59-63.

Dhoka MVand Chopade SS (2012). Method development & comparative statistical evaluation of HPLC & HPTLC method for simultaneous estimation of cefadroxilmonohydrate & ambroxol hydrochloride. Indo Global J Pharm Sci. 2(2): 203-212.

El-Gindy A, El-Walily AF and Bedair MF (2000). First-derivative spectrophotometric and LC determination of cefuroxime and cefadroxil in urine. J Pharm Biomed Anal. 23(2-3): 341-352.

ICH Q2 (R1)(2005). Validation of Analytical Procedures: Text and Methodology. International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human use, ICH Harmonised Tripartite Guideline.

IndianPharmacopoeia (2010).TheIndianPharmacopoeia Commission, Ghaziabad, Govt. of India, Ministry of Health and Family Welfare, Volume-II, pp.998-1003.

IndianPharmacopoeia (2010). The IndianPharmacopoeia Commission, Ghaziabad, Govt. of India, Ministry of Health and Family Welfare, Volume-III, pp.1965-1966.

Jain D, Jain DK and Trivedi PK (1998).Simultaneous spectro-photometric determination of amoxicillin and probenecid in tablet dosage form. Ind J Pharm Sci. 1: 318-320.

Jain D, Jain DK and Trivedi PK (1997).Derivative spectro-photometric method for simultaneous estimation of cephalexin and probenecid in two component solid dosage form. Ind J Pharm Sci. 1: 324 -327.

Katzung BG (2007). Basic & Clinical Pharmacology, 10th ed. The Mc Graw Hill Publishing Company Limited, New Delhi, pp 726-744.

Kurian T, Kurian J, William H and Bijumol C (2012).Simultaneous multicomponent analysis in pharmaceutical formulation by liquid chromatography. Hygeia J D Med. 4(2): 64-48.

Madhuri TR, Spandana M,Aruna D and Susmitha K (2012). Development and validation of new analytical methods for the quantitative estimation of cefadroxil in bulk and pharmaceutical dosage forms. Int J Pharm World Res. 3(2): 1-20.

Patil SV, Patil SD, Patil BF, Patil SP, Tarade VA and Zahid AMJ (2011). Reverse phase HPLC method for analysis of cefadroxil in pharmaceutical dosage form.Int J Pharm Res Dev. 3(6): 155-160.

RangHP, DaleMM, RitterJM and Flower RJ (2003). Rang and Dale Pharmacology, 5th ed. Elsevier Publication, pp 254-255.

Shinde VM, Desai BS and Tendolkar NM (1994). Simultaneous determination of cephalexin & probenecid from tablets by RP-HPLC.Ind J Pharm Sci. 56(2): 58-60.

Sireesha KR, Mhaske DV, Kadam SS and Dhaneshvar SR (2004). Development and validation of a HPTLC method for simultaneous estimation of cefuroxime axetil and probenecid. Ind J Pharm Sci. 66(3): 278-282.

Suddhasattya D, Kalyani K, Samyuktha B, Sahoo SK, Mohapatra S, Murthy PN and dirham K (2010). Development and validation of a UV-VIS spectrophotometric method for the estimation and degradation monitoring of cefadroxil in bulk and pharmaceutical dosage forms. Int J Chem Res. 1(1): 29-34.