RP-HPLC Assay for Estimation of Pitavastatin in Bulk and Pharmaceutical Dosage Forms

DOI:

https://doi.org/10.37285/ijpsn.2014.7.1.5

Authors

  • K Tirumala
  • CH.V.S Gautam
  • J Gangadhar
  • M Jayajeevitha
  • Vanitha Prakash K

Abstract

Reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the estimation of pitavastatin in tablet dosage form. A Phenomenex Luna C18, 150×4.6 mm i.d, 5 µm particle size with mobile phase consisting of buffer 0.01M potassium dihydrogen ortho phosphate pH (3.75) adjusted with dilute orthophosphoric and acetonitrile in the ratio of 20:80 v/v was used. The flow rate was 1.2 mL/min and eluents were monitored at 248 nm. The retention time was 4.1min. The detector response was linear in the concentration of 25-150 µg/mL, with the regression coefficient of 0.9998. Quantification was done by calculating area of the peak and the limit of detection and limit of quantification were 1.9 µg/mL and 5.7 µg/mL respectively. The percentage assay of pitavastatin was 101.1%. The results of study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate which is useful for the routine determination of pitavastatin in bulk drug and in its pharmaceutical dosage forms.

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Keywords:

HPLC, Pitavastatin, Method validation, RP-HPLC, LOQ

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Published

2014-02-28

How to Cite

1.
Tirumala K, Gautam C, Gangadhar J, Jayajeevitha M, K VP. RP-HPLC Assay for Estimation of Pitavastatin in Bulk and Pharmaceutical Dosage Forms. Scopus Indexed [Internet]. 2014 Feb. 28 [cited 2024 Dec. 10];7(1):2346-9. Available from: https://www.ijpsnonline.com/index.php/ijpsn/article/view/698

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Section

Research Articles

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