Effect of PHE against Trypsin and Egg-albumin Induced Experimental Model of Asthma in Mice

DOI:

https://doi.org/10.37285/ijpsn.2013.6.4.13

Authors

  • Anita A. Mehta
  • P V Gohil

Abstract

The present investigation was carried out to study the effect of PHE in experimental induced bronchial asthma in mice. Trypsin and egg-albumin induced chronic model of asthma was used and various parameters were measured on 35th day. The asthmatic control group showed lower level of pO2, tidal volume, airflow rate and higher respiratory rate, serum bicarbonate level, eosinophil count in bronchoalveolar lavage (BAL) fluid compared to normal control group. Dexamethasone and PHE treated groups showed higher pO2 level, tidal volume, airflow rate whereas lower respiratory rate, serum bicarbonate level, eosinophil count in bronchoalveolar lavage fluid compared to asthmatic control group. Histopathological examination of lungs showed more prominent alveolar and muscular layer destruction in asthmatic control group than dexamethasone and PHE treated groups. PHE has beneficial effect in asthma and might be used for the treatment of bronchial asthma. 

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Keywords:

asthma, trypsin, egg albumin, eosinophil, dexamethasone

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Published

2013-12-31

How to Cite

1.
Mehta AA, Gohil PV. Effect of PHE against Trypsin and Egg-albumin Induced Experimental Model of Asthma in Mice. Scopus Indexed [Internet]. 2013 Dec. 31 [cited 2024 May 18];6(4):2299-304. Available from: https://www.ijpsnonline.com/index.php/ijpsn/article/view/693

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Research Articles

References

Apps MCP (1992). A guide to lung function test. Br J Hosp Med 48(7): 396-401.

Charaka S (1949). Shri Gulabkunverba Ayurvedic Society. Ayurvedic Mundranalaya 4: 33.

Djukanovic R, Roche WR, Wilson JW, Beasley CR, Twentyman OP, Howarth RH and Holgate ST (1990). Mucosal inflammation in asthma. Am Rev Respir Dis 142: 434–457.

Ebeling C, Forsythe P, Ng J, Gordon JR, Hollenberg M and Vliagoftis H (2005). Proteinase activated receptor 2 activation in the airways enhances antigen-mediated airway inflammation and airway hyperresponsiveness through different pathways. J Allergy Clin Immunol 115(3): 623–630.

Fabbri L, Caramori G and Maestrelli P (1997). Definition, clinical features, investigations and differential diagnosis of asthma. In: Allergy and Allergic Diseases (Kay AB ed). National Heart and Lung Institute 1347-1359.

Garg K, Bhal I and Kaul M (1996). A Textbook of Histology, 2nd ed. CBS Publishers and Distributers, India, pp. 152-156.

Ghule ST and Patil DK (2001). Kisan World. 28: 33-34.

Gohil P V and Mehta AA (2011). Evaluation of mast cell stabilizing and anti-anaphylactic activity of polyherbal formulation. Adv Biol Res 5(6): 304-308.

Gohil PV, Mehta KA, Chauhan S, Seth AK, Sharma SS and Mehta AA (2011). Phytochemical and pharmacological screening of novel polyherbal formulations. Asian J Pharmaceut Biol Res 1: 112-122.

Govindan S, Viswanathan S, Vijayasekaran V and Alagappan R (1999). A pilot study on the clinical efficacy of Solanum xanthocarpum and Solanum trilobatum in bronchial asthma. J Ethnopharmacol 66: 205-210.

Guyton A and Hall J (2006). Respiration. In: Textbook of Medical Physiology, 11th ed. Saunder Publication, Pennsylvenia, USA, pp. 471-532.

Kamboj VP (2000). Herbal medicine. Curr Sci 78: 35-39.

Kelly HW and Sorknes CA (2005). Asthma. In: Pharmacotherapy- A Pathophysiological Aproch, (Dipiro JT, Talbert RL, Yee GC, Matzke TR, Wells BG, Posey LM, eds.) 6th ed., The McGraw-Hill, pp: 508.

Kim D, Kim SH, Park EJ, Kang CY, Cho SH and Kim S (2009). Anti-allergic effects of PG102, a water-soluble extract prepared from Actinidia arguta, in a murine ovalbumin-induced asthma model. Clin Exp Allergy 39(2): 280-289.

Milos F and Snezana C (2002). The Roll of Eosinophils in Asthma. Med Biol 8(1): 6-10.

Godkar PB (1996). Acid-base balance. In: Textbook of Medical Laboratory Technology, 11th ed., Bhalani Publishing House, New Delhi, pp. 252-257.

Papiris S, Kotanidou A and Malagari K, Roussos C (2002). Clinical review: severe asthma. Critical Care 6(1): 30-44.

Phillip F (2003). Gene therapy for asthma. Mol Ther 7: 148–152.

Khandpur R (1996). Pulmonary function analyzer. In: Handbook of Biomedical Instrument, 1st ed., Tata McGraw-Hill Publishing Company, New Delhi, pp. 308-333.

Shah S, Shah G and Gohil P (2010). Role of estrogen receptor-α in an experimental model of bronchial asthma. Iranian Biomedical Journal 14: 41-48.

Salib RJ, Drake LA and Howarth PH (2003). Allergic rhinitis: past, present and the future. Clin Otolaryngol 28: 291-303.

Schmidlin F, Amadesi S, Dabbagh K, Lewis DE, Knott P, Bunnett NW, Gater PR, Geppetti P, Bertrand C and Stevens ME (2002). Proteaseactivated receptor 2 mediates eosinophil infiltration and hyperreactivity in allergic inflammation of the airway. J Immunol 169(9): 5315–5321.

Sembulingum K and Sembulingum P (2006). Disturbance of respiration. In: Essential of Medical Physiology, 4th edition. Jaypee Publication, New Delhi, pp. 640-663.

Venegas JG, Winkler T, Musch G, Marcos F, Melo V, Layfield D, Nora T, Fischman AJ, Callahan RJ, Giacomo B and Scott HR (2005). Self-organized patchiness in asthma as a prelude to catastrophic shifts. Nature 434(7034): 777-782.

Vogel H (2002). Drug discovery and evaluation-Pharmacological assays, 2nd ed., Springer-Verlag, Berlin Heidelberg, New York, USA. pp. 351-383.

Wardlaw AJ (2001). Eosinophils trafficking in asthma. Clin Med 1(3): 214-218.