Reverse Phase Ultrafast Liquid Chromatography Method for Simultaneous Estimation of Citicoline Sodium and Piracetam in Tablets



  • Sagar Suman Panda
  • Ravi Kumar B V V
  • Ganeswar Mohanta
  • Jnyanaranjan Panda


A simple, precise and accurate RP-UFLC method was developed for simultaneous determination of citicoline sodium and piracetam in tablets. Separation was carried on an Enable C18G column (250 mm × 4.6 mm i.d., 5 μm) using methanol: water (10 mM TEA) (75:25, v/v) as mobile phase at flow rate of 1 ml/min in isocratic mode. The PDA detection wavelength was 225 nm. The retention time of citicoline sodium and piracetam were 1.985 and 3.007 min, respectively. The method was validated for linearity, specificity, precision, accuracy and robustness as per ICH guidelines. The method was linear in the concentration range of 1.0 –250 µg/ml with correlation coefficients of 0.999 for both the drugs. The LOD and LOQ for citicoline sodium were 0.25 µg/ml and 0.81 µg/ml, respectively, and for piracetam 0.28 µg/ml and 0.93 µg/ml, respectively. The average recoveries for recovery study were found to be in the range of 100.24-101.09% and 100.08-101.05% for citicoline sodium and piracetam, respectively. The R.S.D. values for intraday, interday and system precision were found to be less than 2%. The method was applied successfully for simultaneous estimation of citicoline sodium and piracetam in combined tablet formulation. 


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Citicoline, piracetam, reverse phase-ultra fast liquid chromatography, triethylamine




How to Cite

Panda SS, B V V RK, Mohanta G, Panda J. Reverse Phase Ultrafast Liquid Chromatography Method for Simultaneous Estimation of Citicoline Sodium and Piracetam in Tablets. Scopus Indexed [Internet]. 2013 May 31 [cited 2024 May 18];6(1):1952-7. Available from:



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