Strategies to Improve the Production of Forskolin from Hairy Root Cultures of Coleus forskohlii Briq.

DOI:

https://doi.org/10.37285/ijpsn.2012.5.2.7

Authors

  • Veeresham C
  • C.S. Reddy
  • Praveena Ch

Abstract

The aim of this study was to elucidate the effect of elicitors and precursors on the production of forskolin from the hairy root cultures of Coleus forskohlii Briq. Hairy root cultures were established from leaf explants by infecting with Agrobacterium rhizogenes strain A4 on MS basal medium. Suspension cultures of hairy root cultures were initiated in MS medium containing IBA (1.0 mg/L), casein hydrolysate (600 mg/L). We investigated the growth of biomass and forskolin production in suspension cultures of hairy roots. The production of forskolin was parallel to the growth of biomass. The maximum production of forskolin was observed after 5 weeks. With the objective to increase the yield of forskolin, abiotic elicitors such as salicylic acid (100 μM and 500 μM), copper sulphate (100 μM and 500 μM), methyl jasmonate (100 μM and 500 μM) and precursors such as α-ketoglutaric acid (0.2 mM and 1.0 mM), L-phenylalanine (0.2 mM and 1.0 mM) were added to hairy root cultures on different days of incubation period and evaluated their effects on production of forskolin. Elicitor, methyl jasmonate (500 μM) and the precursor, L-phenylalanine (1 mM) on day-14 addition significantly enhanced the production of forskolin over the control hairy root cultures C. forskohlii. Given forskolin’s limited commercial supply, this study provides avenues for improving the production of forskolin in the hairy root culture of C. forskohlii.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Keywords:

C. forskohlii, elicitors, forskolin, hairy roots, precursors

Downloads

Published

2012-08-31

How to Cite

1.
C V, Reddy C, Ch P. Strategies to Improve the Production of Forskolin from Hairy Root Cultures of Coleus forskohlii Briq. Scopus Indexed [Internet]. 2012 Aug. 31 [cited 2024 Dec. 11];5(2):1720-6. Available from: https://www.ijpsnonline.com/index.php/ijpsn/article/view/577

Issue

Section

Research Articles

References

Ammon HP and Kemper FH (1982). Ayurveda: 3000 years of traditional medicine. Med Welt. 32: 148-153.

Ammon HP and Muller AB (1985). Forskolin: from an ayurvedic remedy to a modern agent. Planta Med, 46: 473-477.

Archana G, Ravindra ST, Dhingra V, and Lakshmi Narasu M(2001). Influence of different strains of Agrobacterium rhizogenes on induction of hairy-roots and artemisinin production in Artemisia annua. Curr Sci 81: 378-382.

Balaji K, Veeresham C, Srisilam K, and Kokate CK (2003). Azadirachtin, a novel biopesticide form cell cultures of Azadirachta indica. J. Plant Biotech. 5: 121-129.

Ballica R, Ryu YDD, and Kado IC (1993). Tropane alkaloid production in Datura stramonium suspension cultures: Elicitor and precursor effects. Biotech. Bioengg. 41: 1075-1081.

Banerjee S, Rahman L, Uniyal GC, and Ahuja PS (1998). Enhanced production of valepotriates by Agrobacterium rhizogenes induced hairy root cultures of Valeriana wallichii DC. Plant Sci. 131: 203–208.

Bhandra R, and Shanks JV (1997). Transient studies of nutrient uptake, growth and indole alkaloid accumulation in heterotrophic cultures of hairy toot cultures of Catharanthus roseus. Biotechnol Bioeng. 55: 527-534.

Byrne M, Mc Donnell R, Wright M, and Carnes M (1987). Strain and genotype specificity in Agrobacterium soyabean interaction. Plant Cell Tissue Organ Cult. 8: 3-5.

Charlwood BV and Charlwood KA (1991). Terpenoid production in plant cell culture. In: Harborne JB and Tomas-Barberan FA (eds.), Ecological chemistry and biochemistry of plant terpenoids. Oxford: Clarendon Press, 95-132.

Delpech B, Calvo D, and Lett R (1996). Total synthesis of forskolin. Tetrahedron Lett. 37: 1015-1018.

DeSouza NJ and Shah V (1988). Forskolin – An adenylate cyclase activating drug from an Indian herb. In: Economic and Medicinal Plant research, (eds., Wagner H, Hikino H and Fransworth NR), Academic Press, New York, pp.1-16.

Fett-Neto AG, Melanso SJ, Nicholson SA, Pennington JJ, and Dicosmo F (1994b). Improved taxol yield of aromatic carboxylic acid and amino acid feeding to cell cultures of Taxus cuspidata. Biotech. Bioeng. 44: 967-971.

Fett-Neto AG, Zhang WY, and Dicosmo F (1994a). Kinetics of Taxol production, growth and nutrient uptake in cell suspension of Taxus cuspidata. Biotech. Bioeng. 44: 205-210.

Flores HE and Filner P (1985). Metabolic relationships of putrescine, GABA, and alkaloids in cell and root cultures of Solanaceae. In: Neumann KH, Barz W, Reinhard E (eds.) Primary and secondary metabolism of plant cell cultures. Berlin: Springer-Verlag, 174-186.

Flores HE, Hog MW, and Pickard JJ (1987). Secondary metabolites from root cultures. Trends Biotech. 5: 64-69.

Flores HE, Vivanco JM, and Loyola-Vargas VM (1999). Radical biochemistry: the biology of root-specific metabolism. Trends Plant Sci. 4: 220–226.

Furmanowa M, Gownia K, Zobel A, Guzewska J, Zgorka G, Rapczewska L, and Jozefczyk A (1995). Taxol in Taxus baccata L. var. elegant issima organs and in tissue culture. Med. Fac. Landbouww. Univ. Gent. 60: 2115-2118.

Inamadar PK, Kanitkar PV, Reden J, and DeSouza NJ (1984). Quantitative determination of forskolin by TLC and HPLC. Planta Medica 50: 30-34.

Kittipongpatana N, Hock RS, and Porter JR (1998). Production of solasodine by hairy root, callus, and cell suspension cultures of Solanum aviculare Forst. Plant Cell Tiss. Organ Cult. 52: 133-143.

Krombholz R, Mersinger R, Kries W, and Reinhard E (1992). Production of forskolin by axenic Coleus forskohlii roots cultivated in shake flasks and 20 L glass jar bioreactors. Planta Medica. 58: 328-333.

Kuroyangi M, Arakava T, Mikami Y, Yoshida K, Kawahar N, Hayashi T, and Ishimaru H (1998). Phytoalexins from hairy root cultures of Hyoscyamus albus treated with methyl jasmonate. J. Nat. Prod. 61: 1516-1519.

Lee TK, Yamakawa T, Kodama T, and Shimomura K (1998). Effect of chemicals on alkaloid production by transformed roots of Belladonna. Phytochemistry. 49: 2343-2347.

Lindsey K (1986). Incorporation of 14C phenylalanine and 14C cinnamic acid into a capsaicin in cultured cells of Capsicum frutescens. Phytochemistry. 25: 2793-2800.

Masanori K, Arakawa T, Mikami Y, Yoshida K, Kawahar N, Hayashi T, and Ishimaru H (1998). Phytoalexins from hairy roots of Hyoscyamus albus treated with methyl jasmonate and copper sulphate. J. Nat. Prod. 61: 1516-1519.

Mersinger R, Dornauer H, and Reinhard E (1988). Formation of forskolin by suspension cultures of Coleus forskohlii. Planta Medica. 54: 200-203.

Mukherjee S, Ghosh B, and Jha S (1996). Forskolin synthesis in in vitro cultures of Coleus forskohlii Briq transformed with Agrobacterium tumefaciens. Plant Cell Rep. 15: 691-694.

Mukherjee S, Ghosh B, and Sumita J (2007). Establishment of forskolin yielding transformed cell suspension cultures of Coleus forskohlii as controlled by different factors. J. Biotech. 76: 73-81.

Parry AD, Tiller SA, and Edwards R (1994). The effects of heavy metals and root immersion on isoflavonoid in alfalfa (Medicago sativa L). Plant Physiol. 106: 195-202.

Payne GF, Bringi V, Prince C, and Shuler ML (eds.) (1992), In Plant cell and tissue culture in liquid systems, Hanser publishers, Munich, Vienna, New York, Barcelona, pp.181.

Petit A, David C, Dahl GA, Ellis JG, and Guyon P(1983). Further extension of the opine concept: Plasmids in Agrobacterium rhizogenes cooperate for opine degradation. Mol.Gen.Gent. 190: 204-214.

Porter JR (1991). Host range and implications of plant infection by Agrobacterium rhizogenes. Crit. Rev. Plant Sci. 10: 387-421.

Raul C, Francesc V, Jaume B, and Carles C (2004). Improved production of Galanthamine and related alkaloids by in Narcissus confuses shoot-clumps. Planta Medica. 70: 1180-1188.

Reddy SP, Rudrege R, and Rajashekaran R (2001). Shoot organogenesis and mass propagation of Coleus forskohlii from leaf derived callus. Plant Cell Tiss. Org. Cult. 66: 183-188.

Rupp RH, De Souza NJ, and Dohdwalla AN (eds.) (1986), Proceedings of The International Symposium on forskolin, Bombay, 28-29th January, 1985.

Sandra I, Pitta Alvarez, Tatiana C, Spollansky, and Giulietti AM (2000). The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzyme and Microb. Tech. 26: 252-258.

Sasaki K, Udagawa A, Ishimaru H, Hyashi T, Alfermann AN, Nakanishi F, and Shimomura K (1998). High Forskolin production in hairy roots of Coleus forskohlii. Plant Cell Rep. 17: 457-459.

Sen J, Sharma AK, Sahu NP, and Mahato SB (1992) . Production of forskolin in in vitro cultures of Coleus forskohlii. Planta Medica. 58: 324-327.

Shah V, Bhat SV, Bajura BS, Dornauer H, and DeSouza NJ (1980). The occurrence of forskolin in labiatae. Planta Medica 39: 183-185.

Shah V (1996). Cultivation and Utilization of Medicinal Plants (Supplement), RRL and CSIR, Jammu-Tawi, 385-411.

Srinivasan V, Ciddi V, Bringi V, and Shuler ML. Metabolic inhibitors, elicitors and precursors as tools for probing yield limitation in Taxane production by Taxus chinensis cell cultures. Biotechnol. Prog. 12: 457-465 (1996).

Stachel SE, Messens E, Van Montagu M, and Zambryski, P (1985). Identification of signal molecules produced by wounded plant cells which activated the T-DNA transfer process in Agrobacterium tumefaciens. Nature. 318: 624- 629.

Sundarvelan C, Brahma RD, and Veeresham C (2002). Effects of elicitors and precursors on production of Camptothecine and its analogus in cell cultures of Nothapodytes foetida. Ethiopian. Pharma. J. 20: 39-46.

Sylvain R, Vincent C, Nicholas PC, Benoit SP, and Vincenzo DL (2003) . Jasmonate induced epoxidation of tabersonine by a cytochrome P-450 in hairy root cultures of Catharanthus roseus. Phytochemistry. 64: 401-409.

Toivonen L, Ojala M, and Kauppinen V (1990). Indole alkaloid production by hairy root cultures Catharanthus roseus: growth kinetics and fermentation. Biotechnol Lett. 12: 519-524.

Trevelyan WE, Procter DP, and Harrison JP (1950). Detection of sugars on paper chromatograms. Nature. 166: 444-445.

Vanisree M, Lee CY, Lo SF, Nalawade SM, Lin CY, and Tsay HS (2004). Studies on the production of some important secondary metabolites from medicinal plants by plant tissue cultures. Bot. Bull. Acad. Sin. 45: 1-22.

Vishwakarma RA, Tyagi BR, Ahmed B, and Hussain (1998). Variation in forskolin content in the roots of C. forskohlii. Planta Medica. 5: 471-472.

Veeresham C, Kokate CK, and Venkateswarlu V (1994). Influence of precursors on production of isoquinoline alkaloids in tissue cultures of Cephaelis ipecacuanha. Phytochemistry 35: 947-949.

Veeresham C, Mamatha R, Prasad Babu CH, Srisilam K, and Kokate CK (2003) . Production of taxol and its analogues from cell cultures of Taxus wallichiana. Pharmaceutical Biol. 41: 426-430.

Yukimune Y, Tabata H, Higashi Y, and Hara Y (1996). Methyl jasmonate induced over production of paclitaxel and baccatin-III in Taxus cell suspension cultures. Nature Biotech. 14: 1129-1132.

Zabetakis I, Edwards R, and Hugan DO (1999). Elicitation of tropane alkaloid biosynthesis in transformed root cultures of Datura Stramonium. Phytochemistry 50: 53- 56.

Zehra M, Banerjee S, Sharma S, and Kumar S (1999). Influence of Agrobacterium rhizogenes strains on biomass and alkaloid productivity in hairy root lines of Hyoscyamus muticus and H. albus. Planta Medica 65: 60-63.

Zhouli G, Hu H, Yang CR, and Wang J (1996). Formation of labdane diterpenoids by hairy root cultures of Coleus forskholii. Acta Botanica Yunnanica 18: 445-450.