Development and Validation of Simple UV Spectrophoto-metric Method for Estimation of Azithromycin in Dosage Form using Hydrotropic Solubilization Technique

DOI:

https://doi.org/10.37285/ijpsn.2023.16.6.5

Authors

  • SURYA PRAKASH GUPTA Rajiv Gandhi Institute of Pharmacy,Faculty of Pharmaceutical Science & Technology AKS University, Satna
  • ABHISHEK SONI Rajiv Gandhi Institute of Pharmacy,Faculty of Pharmaceutical Science & Technology AKS University, Satna

Abstract

Objective: A new UV method was developed for the estimation of azithromycin in tablets using the hydrotropic solubilization method and it was validated as per ICH guidelines. 

Methods: The solubility of azithromycin was studied in various hydrotropic agents and the one with the best solubility was considered as solvent for developing and validating the UV spectrophotometric method.

Results: The best solubility of azithromycin was witnessed in a 2M sodium acetate solution in water. The absorption maximum of azithromycin in 2M sodium acetate was found to be 221 nm. The method was found to be linear in the range of 10-50µg/ml. The precision of the method was good and the recovery of drugs was well within the acceptance limits of 80-120%. 

Conclusion: The proposed UV method was found suitable for the estimation of azithromycin in formulations and is simple, selective, reproducible, and accurate with good precision, and can be successfully applied to routine analytical purposes.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Keywords:

Azithromycin, estimation, UV-Visible spectroscopy, hydrotropy, ICH, validation

Downloads

Published

2023-11-15

How to Cite

1.
GUPTA SP, ABHISHEK SONI. Development and Validation of Simple UV Spectrophoto-metric Method for Estimation of Azithromycin in Dosage Form using Hydrotropic Solubilization Technique. Scopus Indexed [Internet]. 2023 Nov. 15 [cited 2024 Dec. 11];16(6):7057-61. Available from: https://www.ijpsnonline.com/index.php/ijpsn/article/view/3070

Issue

Section

Research Articles

References

Azithromycin. Available from https://www.drugbank.ca/drugs/DB00207; assessed on 06/12/2021

Azithromycin. Indian Pharmacopoeia, Volume 2. Ministry of Health and Family Welfare, Govt of India.2007 (pp 140-141).

Al-Rimawi F, Kharoaf M. Analysis of azithromycin and its related compounds by RP-HPLC with UV detection. Journal of Chromatographic Science. 2010; 48: 86-90

Bhimani S, Sanghvi G, Pethani T, Dave G, Airao V, Sharma T, Sheth N, Vaishnav D. Development of the UV spectrophotometric method of azithromycin in API and stress degradation studies. International Letters of Chemistry, Physics and Astronomy. 2016; 68: 49-56

Dewan I, Amin T, Hossain MF, Hasan M, Chowdhury SF, Gazi M, Ashraful Islam SM. Development and validation of a new HPLC method for the estimation of azithromycin in bulk and tablet dosage form. International Journal of Pharmaceutical Sciences Research. 2013; 4: 282-286

Ghari T, Kobarfard F, Mortazavin SA. Development of a Simple RP-HPLC-UV Method for Determination of Azithromycin in Bulk and Pharmaceutical Dosage forms as an Alternative to the USP Method. Iranian Journal of Pharmaceutical Research. 2013; 12: 57-60

Jayanna BK, Nagendrappa G, Arunkumar, Gowda N. Spectrophotometric estimation of azithromycin in tablets. Indian Journal of Pharmaceutical Sciences. 2012; 74: 365-369

Nyola N, Jeyabalan GS. Simultaneous estimation of Azithromycin and Cefixime in Active Pharmaceutical Ingredients and Pharmaceutical dosage forms by Spectrophotometry.‏ Hygeia Journal for drugs and medicines. 2013; 4: 27-32

Suma B, Thachemperil JM, Venkataramana C. Spectrophotometric method development and validation of azithromycin in tablet formulation. Journal of Dental Orofacial Research. 2018; 14: 23-27

Maheshwari RK, Maheshwari RB, Bhatt P. Simultaneous Spectrophotometric Estimation of Norfloxacin and Tinidazole in Two Component Tablet Formulation. Asian Journal of Chemistry. 2006; 18: 1481-1486.

Maliwal D, Jain A, Maheshwari RK, Patidar V. Simultaneous Spectrophotometric Estimation of Metronidazole and Norfloxacin in Combined Tablet Formulation Using Hydrotropy. Research Journal of Pharmacy and Technology. 2008; 1(4): 357-361.

SharmaR, Pathodiya GT, Mishra GP, Sainy J. Spectrophotometric methods for simultaneous estimation of Paracetamol and Diclofenacsolidum in Combined Dosage formby application of Hydrotropic Solubilization. Journal of Pharmaceutical Sciences and Research. 2010; 2(12):821-826.

Biswas P, Jain P, Parkhe G, Mishra B. Validated HPLC method for the estimation of Aspirin and Omeprazole in dosage form. Journal of Pharmacology and Biomedicine. 2018; 2(3): 171-180.

Pandey K, Jain P, Mishra B. Estimation of Diacerin using hydrotropic agent and dye drug interaction. Journal of Pharmacology and Biomedicine. 2017; 1(2): 85-93.