Green Synthesis and Characterization of Silver Nanoparticles using Glycine Max L. Seed Extract and their Antiepileptic Activity in Rats
DOI:
https://doi.org/10.37285/ijpsn.2017.10.6.6Abstract
Glycine max is an edible oil seed and commonly known as Soybean. In this study, we report green, inexpensive method for synthesis of silver nanoparticles (SNPs) using of Glycine max seeds aqueous extract. Synthesized SNPs were characterized by UV-Visible spectroscopy (UV), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Zeta Potential. Formation of Silver Nanoparticles from Glycine max Aqueous Extract (SNGMAE), indicated by appearance of dark brown color. UV depicted peak at 440nm, FTIR showed various functional groups present in plant extract capping in Silver Nanoparticles. Nanoparticle range of SNGMAE was identified by SEM and XRD, while stability was confirmed by Zeta Potential. Different constituents of aqueous extract viz., proteins, phytosterols, phospholipids, oils and carbohydrates are involved in the formation of SNPs. Synthesized SNGMAE was reconnoitered for antiepileptic activity in pentylenetetrazol (PTZ) and maximal electroshock induced epileptic (MESE) models, phenytoin (25mg/kg) was used as standard anti-epileptic drug. In negative control group of PTZ, all five stages of convulsions were observed, which were significantly reduced with phenytoin and with SNGMAE at 80 and 160mg/kg. MESE was characterized by appearance of flexion, extensor and clonus. In negative control group, these stages appeared for longer duration; these were decreased significantly in the treatment groups. In conclusion, these results show that SNGMAE may be a potent source of nanomedicine for the treatment of epilepsy.
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Keywords:
Glycine max, Silver nanoparticles, Pentylenetetrazole, Maximal electro shock, Wistar rats, Epilepsy
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Published
2017-11-30
How to Cite
1.
Md IU, D K, N T, A M, A S, J A, et al. Green Synthesis and Characterization of Silver Nanoparticles using Glycine Max L. Seed Extract and their Antiepileptic Activity in Rats. Scopus Indexed [Internet]. 2017 Nov. 30 [cited 2024 May 18];10(6):3909-14. Available from: http://www.ijpsnonline.com/index.php/ijpsn/article/view/873
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Research Articles
References
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Bansal V, Bharde A, Ramanathan R and Bhargava SK (2012). Inorganic materials using ‘unusual’ microorganisms. Adv Colloid Interface Sci 179-182: 150–168.
Barbara G. Wells, Joseph T. Dipiro, Terry L. Schwinghammer, Cecily V. DiPiro as authors of pharmacotherapy handbook , seventh edition & Bertram G. Katzung as author of book of Basic and Clinical Pharmacology, Tenth Edition, 2007.
Barnes S, Peterson TG and Coward L (1995). Rationale for the use of genistein-containing soy matrices in chemoprevention trials for breast and prostate cancer. J Cell Biochem 22: 181-187.
Borowicz KK, Luszczki J and Czuczwar SJ (2002). 2-Chloroadenosine, a preferential agonist of adenosine A1 receptors, enhances the anticonvulsant activity of carbamazepine and clonazepam in mice. Eur Neuropsychopharmacol 12: 173-179.
Brajesh K, Kumari S, Luis C and Alexis D (2014). Synthesis of silver nanoparticles using Sacha inchi (Plukenetia volubilis L.) leaf extracts. Saudi J Biol Sci 21: 605-609.
Calderon FJ, Mikha MM, Vigil MF, Nielsen DC, Benjamin JG and Reeves III JB (2011). Diffuse-reflectance mid-infrared spectral properties of soils under alternative crop rotations in a semi-arid climate. Commun Soil Sci Plan 42: 2143-2159.
Dahanukar SA and Kulkarni AR (2000). Pharmacology of medicinal plants and natural products. Indian J. Pharmacol 32: 81-118.
Ekici F, Gurol G and Ateş N (2014). Effects of linoleic acid on generalized convulsive and non-convulsive epileptic seizures. Turk J Med Sci 44: 535-539.
Elterman RD, Shields WD, Mansfield KA and Nakagawa J (2001). US Infantile Spasms Vigabatrin Study Group. Randomized trial of vigabatrin in patients with infantile spasms. Neurology 57: 1416-21
Gole A, Dash C and Ramakrishnan V (2001). Pepsin-gold colloid conjugates: preparation, characterization, and enzymatic activity. Langmuir 17: 1674-1679.
Grieshop CM, Kadzere CT, Clapper GM, Flickinger EA, Bauer LL, Frazier RL, et al (2003). Chemical and nutritional characteristics of United States soybeans and soybean meals. J Agric Food Chem 51: 7684-91.
Huang J, Zhan G, Zheng B, Sun D, Lu F, Lin Y, et al (2011). Biogenic silver nanoparticles by Cacumen platycladi extract: synthesis, formation mechanism, and antibacterial activity. Ind Eng Chem Res 50: 9095-9106.
Jeyaraj M, Satish KG, Sivanandan G, Mubarak AD, Rajesh M, Arun R, et al (2013). Biogenic silver nanoparticles for cancer treatment: an experimental report. Colloids surf B Biointerfaces 106: 86-92.
Jibu T, Subha MV and Edgar JV (2012). Antidiabetic and Antihyperlipidemic Activity of the Extracts of the Seeds of Glycine max (L) in Streptozotocin Induced Diabetic Mice. Drug Invent Today 4: 677-680.
Korbekandi H, Iravani S. Silver nanoparticles, the delivery of nanoparticles. In: Hashim Abbass A., editor, ISBN: 978-953- 51-0615-9, InTech; 2012.
Krithiga N, Rajalaksmi A and Jayachitra A (2015). Green Synthesis of Silver Nanoparticles Using Leaf Extracts of Clitoria ternatea and Solanum nigrumand Study of Its Antibacterial Effect against Common Nosocomial Pathogens. Journal of Nanoscience 1- 8.
Kudou S, Fleury Y, Welti D, Magnolato D, Uchida T, Kitamura K, et al (1991). Malonyl isoflavone glycosides in soybean seeds (Glycine max Merrill). Agric Biol Chem 55: 2227-2233.
Kulkarni SK (2007). Hand book of experimental pharmacology, 3rd ed. Vallabh Prakshan, India.
Kumar V and Yadav SK (2009). Plant-mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol 84: 151-157.
Kwan P and Brodie MJ (2000). Early identification of refractory epilepsy. NEngl J Med 342: 314-319.
Li JL, Gao ZB and Zhao WM (2016). Identification and Evaluation of Antiepileptic Activity of C21 Steroidal Glycosides from the Roots of Cynanchum wilfordii. J Nat Prod 79: 89-97.
Neha N, Ayaz SA and Suboor K (2014). Metal Chelating Activity of Glycine max Seed Extract on Ferrous +Doxorubicin-Induced Cardiotoxicity in Rats. Ann Exp Biol 2: 43-48.
Newton CR and Gtarcia HH (2012). Epilepsy in poor regions of the world. Lancet 380: 1193-1201.
Ozawa Y, Sato H, Nakatani A, Mori O, Hara Y, Nakada Y, et al (2001). Chemical composition of soyabean oil extracted from hypocotyl-enriched soyabean raw material and its cholesterol lowering effects in rats. J Oleo Sci 50: 217-223.
Paramdeep S, Damanpreet S, Rajesh KG (2010). Phytoflavonoids: antiepileptics for the future. Int J Pharm Sci 6: 51-66
Patil SV, Borase HP, Patil CD and Salunke BK (2012). Biosynthesis of silver nanoparticles using latex from few euphorbian plants and their antimicrobial potential. Appl Biochem Biotechnol 167: 776-790.
Rang HP, Dale MM, Ritter JM and Flower RJ (2007). Rang and Dale’s Pharmacology, 6th ed. Churchill Livingstone Elsevier: China.
Rashid MMO, Ferdous J, Banik S, Islam MDR, Mazbahuddin AHM and Robel FN (2016). Anthelmintic activity of silver-extract nanoparticles synthesized from the combination of silver nanoparticles and M.charantia fruit extract. BMC Complem Altern M 16: 242: 1-6.
Sato K, Yamagishi T, Yamauchi F (1986). Quantitative analysis of soyabean proteins by densitometry on gel electrophoresis. Cereal Chem 63: 493-496.
Shankaranarayanan J, Christina AJM, Kalyan SB, Jagan A, Sundara SK and Balakumar M (2009). Evaluation of Glycine max Merill. Seeds for Anti-arthritic Activity in Male Wistar Rats. Int J Pharm Sci Nanotechnol 1: 363-366.
Sharma VK, Yngard RA and Lin Y (2009). Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 145: 83-96.
Singh D, Singh B and Goel RK (2012). Role of saponins for the anticonvulsant effect of adventitious roots of Ficus religiosa. Pharm Biol 50: 816-22.
Singh K, Manju P and Sangeeta K (2014). Green silver nanoparticles of Phyllanthus amarus: as an antibacterial agent against multi drug resistant clinical isolates of Pseudomonas aeruginosa. J Nanobiotechnol 12: 40: 1-9.
Supriya KST and Ramachandra YL (2014). Extraction, Isolation and Antimicrobial Activity of Crude and Purified Ferritin extract from seeds of Soyabean (Glycine max (L.) Merr. ). Sch Acad J Pharm 3: 97-99.
Tello I, Campos-Pena V, Montiel E, Rodriguez V, Aguirre-Moreno A, Leon-Rivera I, et al (2013). Anticonvulsant and Neuroprotective Effects of Oligosaccharides from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) Int J Med Mushrooms 15: 555-68.
Vigneshwaran N, Ashtaputre NM, Varadarajan PV, Nachane RP, Paralikar KM and Balasubramanya RH (2007). Biological synthesis of silver nanoparticles using the fungus Aspergillus flavus. Mater Lett 61: 1413-1418.
Wang ZL (2000). Characterization of Nanophase Materials, 1st ed. Wiley-VCH, Germany.
Watanabe Y, Takechi K, Fujiwara A and Kamei C (2010). Effects of antiepileptics on behavioral and electroencephalographic seizure induced by pentetrazol in mice. J Pharmacol Sci 112: 282-289.
Willner I, Baron R and Willner B (2006). Growing metal nanoparticles by enzymes. Adv Mater 18: 1109-1120.
Bansal V, Bharde A, Ramanathan R and Bhargava SK (2012). Inorganic materials using ‘unusual’ microorganisms. Adv Colloid Interface Sci 179-182: 150–168.
Barbara G. Wells, Joseph T. Dipiro, Terry L. Schwinghammer, Cecily V. DiPiro as authors of pharmacotherapy handbook , seventh edition & Bertram G. Katzung as author of book of Basic and Clinical Pharmacology, Tenth Edition, 2007.
Barnes S, Peterson TG and Coward L (1995). Rationale for the use of genistein-containing soy matrices in chemoprevention trials for breast and prostate cancer. J Cell Biochem 22: 181-187.
Borowicz KK, Luszczki J and Czuczwar SJ (2002). 2-Chloroadenosine, a preferential agonist of adenosine A1 receptors, enhances the anticonvulsant activity of carbamazepine and clonazepam in mice. Eur Neuropsychopharmacol 12: 173-179.
Brajesh K, Kumari S, Luis C and Alexis D (2014). Synthesis of silver nanoparticles using Sacha inchi (Plukenetia volubilis L.) leaf extracts. Saudi J Biol Sci 21: 605-609.
Calderon FJ, Mikha MM, Vigil MF, Nielsen DC, Benjamin JG and Reeves III JB (2011). Diffuse-reflectance mid-infrared spectral properties of soils under alternative crop rotations in a semi-arid climate. Commun Soil Sci Plan 42: 2143-2159.
Dahanukar SA and Kulkarni AR (2000). Pharmacology of medicinal plants and natural products. Indian J. Pharmacol 32: 81-118.
Ekici F, Gurol G and Ateş N (2014). Effects of linoleic acid on generalized convulsive and non-convulsive epileptic seizures. Turk J Med Sci 44: 535-539.
Elterman RD, Shields WD, Mansfield KA and Nakagawa J (2001). US Infantile Spasms Vigabatrin Study Group. Randomized trial of vigabatrin in patients with infantile spasms. Neurology 57: 1416-21
Gole A, Dash C and Ramakrishnan V (2001). Pepsin-gold colloid conjugates: preparation, characterization, and enzymatic activity. Langmuir 17: 1674-1679.
Grieshop CM, Kadzere CT, Clapper GM, Flickinger EA, Bauer LL, Frazier RL, et al (2003). Chemical and nutritional characteristics of United States soybeans and soybean meals. J Agric Food Chem 51: 7684-91.
Huang J, Zhan G, Zheng B, Sun D, Lu F, Lin Y, et al (2011). Biogenic silver nanoparticles by Cacumen platycladi extract: synthesis, formation mechanism, and antibacterial activity. Ind Eng Chem Res 50: 9095-9106.
Jeyaraj M, Satish KG, Sivanandan G, Mubarak AD, Rajesh M, Arun R, et al (2013). Biogenic silver nanoparticles for cancer treatment: an experimental report. Colloids surf B Biointerfaces 106: 86-92.
Jibu T, Subha MV and Edgar JV (2012). Antidiabetic and Antihyperlipidemic Activity of the Extracts of the Seeds of Glycine max (L) in Streptozotocin Induced Diabetic Mice. Drug Invent Today 4: 677-680.
Korbekandi H, Iravani S. Silver nanoparticles, the delivery of nanoparticles. In: Hashim Abbass A., editor, ISBN: 978-953- 51-0615-9, InTech; 2012.
Krithiga N, Rajalaksmi A and Jayachitra A (2015). Green Synthesis of Silver Nanoparticles Using Leaf Extracts of Clitoria ternatea and Solanum nigrumand Study of Its Antibacterial Effect against Common Nosocomial Pathogens. Journal of Nanoscience 1- 8.
Kudou S, Fleury Y, Welti D, Magnolato D, Uchida T, Kitamura K, et al (1991). Malonyl isoflavone glycosides in soybean seeds (Glycine max Merrill). Agric Biol Chem 55: 2227-2233.
Kulkarni SK (2007). Hand book of experimental pharmacology, 3rd ed. Vallabh Prakshan, India.
Kumar V and Yadav SK (2009). Plant-mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol 84: 151-157.
Kwan P and Brodie MJ (2000). Early identification of refractory epilepsy. NEngl J Med 342: 314-319.
Li JL, Gao ZB and Zhao WM (2016). Identification and Evaluation of Antiepileptic Activity of C21 Steroidal Glycosides from the Roots of Cynanchum wilfordii. J Nat Prod 79: 89-97.
Neha N, Ayaz SA and Suboor K (2014). Metal Chelating Activity of Glycine max Seed Extract on Ferrous +Doxorubicin-Induced Cardiotoxicity in Rats. Ann Exp Biol 2: 43-48.
Newton CR and Gtarcia HH (2012). Epilepsy in poor regions of the world. Lancet 380: 1193-1201.
Ozawa Y, Sato H, Nakatani A, Mori O, Hara Y, Nakada Y, et al (2001). Chemical composition of soyabean oil extracted from hypocotyl-enriched soyabean raw material and its cholesterol lowering effects in rats. J Oleo Sci 50: 217-223.
Paramdeep S, Damanpreet S, Rajesh KG (2010). Phytoflavonoids: antiepileptics for the future. Int J Pharm Sci 6: 51-66
Patil SV, Borase HP, Patil CD and Salunke BK (2012). Biosynthesis of silver nanoparticles using latex from few euphorbian plants and their antimicrobial potential. Appl Biochem Biotechnol 167: 776-790.
Rang HP, Dale MM, Ritter JM and Flower RJ (2007). Rang and Dale’s Pharmacology, 6th ed. Churchill Livingstone Elsevier: China.
Rashid MMO, Ferdous J, Banik S, Islam MDR, Mazbahuddin AHM and Robel FN (2016). Anthelmintic activity of silver-extract nanoparticles synthesized from the combination of silver nanoparticles and M.charantia fruit extract. BMC Complem Altern M 16: 242: 1-6.
Sato K, Yamagishi T, Yamauchi F (1986). Quantitative analysis of soyabean proteins by densitometry on gel electrophoresis. Cereal Chem 63: 493-496.
Shankaranarayanan J, Christina AJM, Kalyan SB, Jagan A, Sundara SK and Balakumar M (2009). Evaluation of Glycine max Merill. Seeds for Anti-arthritic Activity in Male Wistar Rats. Int J Pharm Sci Nanotechnol 1: 363-366.
Sharma VK, Yngard RA and Lin Y (2009). Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 145: 83-96.
Singh D, Singh B and Goel RK (2012). Role of saponins for the anticonvulsant effect of adventitious roots of Ficus religiosa. Pharm Biol 50: 816-22.
Singh K, Manju P and Sangeeta K (2014). Green silver nanoparticles of Phyllanthus amarus: as an antibacterial agent against multi drug resistant clinical isolates of Pseudomonas aeruginosa. J Nanobiotechnol 12: 40: 1-9.
Supriya KST and Ramachandra YL (2014). Extraction, Isolation and Antimicrobial Activity of Crude and Purified Ferritin extract from seeds of Soyabean (Glycine max (L.) Merr. ). Sch Acad J Pharm 3: 97-99.
Tello I, Campos-Pena V, Montiel E, Rodriguez V, Aguirre-Moreno A, Leon-Rivera I, et al (2013). Anticonvulsant and Neuroprotective Effects of Oligosaccharides from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) Int J Med Mushrooms 15: 555-68.
Vigneshwaran N, Ashtaputre NM, Varadarajan PV, Nachane RP, Paralikar KM and Balasubramanya RH (2007). Biological synthesis of silver nanoparticles using the fungus Aspergillus flavus. Mater Lett 61: 1413-1418.
Wang ZL (2000). Characterization of Nanophase Materials, 1st ed. Wiley-VCH, Germany.
Watanabe Y, Takechi K, Fujiwara A and Kamei C (2010). Effects of antiepileptics on behavioral and electroencephalographic seizure induced by pentetrazol in mice. J Pharmacol Sci 112: 282-289.
Willner I, Baron R and Willner B (2006). Growing metal nanoparticles by enzymes. Adv Mater 18: 1109-1120.
