Evaluation of Anticancer Activity and Structural Analysis of Biosynthesized Silver Nanoparticles (AgNPs) from Centella Asiatica

DOI:

https://doi.org/10.37285/ijpsn.2024.17.2.7

Authors

  • RUPA R Department of Biotechnology, Sri Shakthi Institute of Engineering & Technology, Coimbatore
  • PAVITHRA B Department of Biotechnology, Sri Shakthi Institute of Engineering & Technology, Coimbatore
  • KAVYA M Department of Biotechnology, Sri Shakthi Institute of Engineering & Technology, Coimbatore
  • NIKHITHA K V Department of Biotechnology, Sri Shakthi Institute of Engineering & Technology, Coimbatore
  • NIVETHA J D Department of Biotechnology, Sri Shakthi Institute of Engineering & Technology, Coimbatore
  • Bindhu J Department of Biotechnology, Sri Shakthi Institute of Engineering & Technology, Coimbatore

Abstract

Introduction: The synthesis of silver nanoparticles (AgNPs) from an ethanolic extract of Centella asiatica leaf, which exhibits anticancer characteristics, was investigated in this study. Green synthesis, a non-harmful procedure for the environment, aids in the production of nanoparticles from Centella asiatica extract. Centella asiatica has long been regarded to have beneficial properties for neurological function, anti-inflammation, and wound treatment.

Objective: FT-IR, GC-MS, and thin-layer chromatography were used to find the phytochemicals present in the extract, and the anti-cancer abilities of the nanoparticles were tested against the PC3 cell line.

Methods: Soxhlet extraction is used to extract the bioactive, and then nanoparticles are synthesised. Following that, they are subjected to preliminary phytochemical analysis and thin-layer chromatography. These can assess the presence or absence of phytochemicals. Soon after, GCMS is done to reveal the molecular weight of the bioactive compounds using the charge-to-mass ratio. The structural peaks of different functional groups were identified using FT-IR. Under different concentrations, the silver nanoparticles were portrayed against the PC3 cell line of prostate cancer.

Result: The concentration of silver nanoparticles was shown to negatively affect cell viability. The IC50 value was 11.8 µg/ml, showing that the inhibition of growth increased with increasing doses of the AgNPs.

Conclusion: Our study highlights the significance of green synthesis of AgNPs from Centella asiatica extract, with bioactive compounds identified using GC-MS showing potential as anticancer agents against PC3 cells, suggesting a promising avenue for cancer therapy.

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Keywords:

Centella asiatica, FTIR, GCMS, Cytotoxicity, PC3, Nanoparticle synthesis

Published

2024-03-31

How to Cite

1.
R R, B P, M K, K V N, J D N, J B. Evaluation of Anticancer Activity and Structural Analysis of Biosynthesized Silver Nanoparticles (AgNPs) from Centella Asiatica. Scopus Indexed [Internet]. 2024 Mar. 31 [cited 2024 Jun. 14];17(2):7256-69. Available from: http://www.ijpsnonline.com/index.php/ijpsn/article/view/3842

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Section

Research Articles

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