In-Vitro 3-Dimensional Organoid Models – A Concise Review on Clinical Importance

DOI:

https://doi.org/10.37285/ijpsn.2024.17.1.8

Authors

  • Ritu Bairagi Assistant Professor, Pharmaceutical chemistry,P. R. Pote Patil College of Pharmacy, Amravati
  • Rupali Patil Assistant Professor, Pharmaceutical chemistry,P. R. Pote Patil College of Pharmacy, Amravati

Abstract

Organoids are three-dimensional structures that are grown in vitro. Adult stem cells, as well as embryonic stem cells and induced pluripotent stem cells, can be used to make them. To derive these organoids requirements are Tissue and Matrix. The matrix gives support and it should resemble the extracellular matrix for the cells. Microcontact printing and Bioprinting techniques are used for preparing matrices. Many organoids are generated from cells like brain organoids, liver organoids, mammary gland organoids, and some cancer organoids. These Organoid models are used for studying the human-specific disease mechanisms. Due to the specific organoid models, study of many tissues is possible ranging from small intestine, prostate, brain and liver. This review explores the applications of cell-derived organoids, their advantages, and current breakthroughs in organoid models.

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Keywords:

Advantages of organoids, Brain organoids, Cancer research, tissue modelling, Vascularization, Organoids, Organoid models

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Published

2024-02-01

How to Cite

1.
Bairagi R, Patil R. In-Vitro 3-Dimensional Organoid Models – A Concise Review on Clinical Importance. Scopus Indexed [Internet]. 2024 Feb. 1 [cited 2024 May 3];17(1):7183-90. Available from: http://www.ijpsnonline.com/index.php/ijpsn/article/view/2877

Issue

Vol. 17 No. 1 (2024): January-February 2024

Section

Review Articles
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Scopus
Google Scholar
Europe PMC

References

Kim J, Koo BK, Knoblich JA. Human organoids: model systems for human biology and medicine. Nature Reviews Molecular Cell Biology. 2020 Oct;21(10):571-84.

Rossi G, Manfrin A, Lutolf MP. Progress and potential in organoid research. Nature Reviews Genetics. 2018 Nov;19(11):671-87.

Schutgens F, Clevers H. Human organoids: tools for understanding biology and treating diseases. Annual Review of Pathology: Mechanisms of Disease. 2020 Jan 24;15:211-34.

John T, Kohler D, Pintilie M, Yanagawa N, Pham NA, Li M, Panchal D, Hui F, Meng F, Shepherd FA, Tsao MS. The ability to form primary tumor xenografts is predictive of increased risk of disease recurrence in early-stage non–small cell lung cancer. Clinical cancer research. 2011 Jan 1;17(1):134-41.

Lancaster MA, Knoblich JA. Organogenesis in a dish: modeling development and disease using organoid technologies. Science. 2014 Jul 18;345(6194):1247125.

Huch M, Koo BK. Modeling mouse and human development using organoid cultures. Development. 2015 Sep 15;142(18):3113-25.

Rozich NS, Blair AB, Burkhart RA. Organoids: a model for precision medicine. In Precision Medicine for Investigators, Practitioners and Providers 2020 Jan 1 (pp. 123-129). Academic Press.

Weiswald LB, Bellet D, Dangles-Marie V. Spherical cancer models in tumor biology. Neoplasia. 2015 Jan 1;17(1):1-5.

Inch WR, McCredie JA, Sutherland RM. Growth of nodular carcinomas in rodents compared with multi-cell spheroids in tissue culture. Growth. 1970 Sep;34(3):271-82.

Kaushik G, Ponnusamy MP, Batra SK. Concise review: current status of three-dimensional organoids as preclinical models. Stem Cells. 2018 Sep;36(9):1329-40.

Perl A, Reinhoudt DN, Huskens J. Microcontact printing: limitations and achievements. Advanced Materials. 2009 Jun 12;21(22):2257-68.

Murphy SV, Atala A. 3D bioprinting of tissues and organs. Nature biotechnology. 2014 Aug;32(8):773-85.

Weinander R, Anderson C, Morgenstern R. Identification of N-acetylcysteine as a new substrate for rat liver microsomal glutathione transferase. A study of thiol ligands. Journal of Biological Chemistry. 1994 Jan 7;269(1):71-6.

Qian X, Song H, Ming GL. Brain organoids: advances, applications and challenges. Development. 2019 Apr 15;146(8):dev166074.

Smits LM, Schwamborn JC. Midbrain organoids: a new tool to investigate Parkinson’s disease. Frontiers in Cell and Developmental Biology. 2020 May 19;8:359.

Kim J, Koo BK, Knoblich JA. Human organoids: model systems for human biology and medicine. Nature Reviews Molecular Cell Biology. 2020 Oct;21(10):571-84.

Prior N, Inacio P, Huch M. Liver organoids: from basic research to therapeutic applications. Gut. 2019 Dec 1;68(12):2228-37.

Xu H, Lyu X, Yi M, Zhao W, Song Y, Wu K. Organoid technology and applications in cancer research. Journal of hematology & oncology. 2018 Dec;11(1):1-5.

Rodrigues J, Heinrich MA, Teixeira LM, Prakash J. 3D in vitro model (R) evolution: unveiling tumor–stroma interactions. Trends in cancer. 2021 Mar 1;7(3):249-64.

Wynn TA, Chawla A, Pollard JW. Macrophage biology in development, homeostasis and disease. Nature. 2013 Apr;496(7446):445-55.

Emerman JT, Pitelka DR. Maintenance and induction of morphological differentiation in dissociated mammary epithelium on floating collagen membranes. In vitro. 1977 May;13(5):316-28.

Sato T, Vries RG, Snippert HJ, Van De Wetering M, Barker N, Stange DE, Van Es JH, Abo A, Kujala P, Peters PJ, Clevers H. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature. 2009 May;459(7244):262-5.

Huch M, Bonfanti P, Boj SF, Sato T, Loomans CJ, Van De Wetering M, Sojoodi M, Li VS, Schuijers J, Gracanin A, Ringnalda F. Unlimited in vitro expansion of adult bi‐potent pancreas progenitors through the Lgr5/R‐spondin axis. The EMBO journal. 2013 Oct 16;32(20):2708-21.

Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA: a cancer journal for clinicians. 2011 Mar;61(2):69-90.

Boj SF, Hwang CI, Baker LA, Chio II, Engle DD, Corbo V, Jager M, Ponz-Sarvise M, Tiriac H, Spector MS, Gracanin A. Organoid models of human and mouse ductal pancreatic cancer. Cell. 2015 Jan 15;160(1-2):324-38.

Heidari-Khoei H, Esfandiari F, Hajari MA, Ghorbaninejad Z, Piryaei A, Baharvand H. Organoid technology in female reproductive biomedicine. Reproductive Biology and Endocrinology. 2020 Dec;18(1):1-9.

Young AN, Moyle-Heyrman G, Kim JJ, Burdette JE. Microphysiologic systems in female reproductive biology. Experimental Biology and Medicine. 2017 Nov;242(17):1690-700.

Chua CW, Shibata M, Lei M, Toivanen R, Barlow LJ, Bergren SK, Badani KK, McKiernan JM, Benson MC, Hibshoosh H, Shen MM. Single luminal epithelial progenitors can generate prostate organoids in culture. Nature cell biology. 2014 Oct;16(10):951-61.

Drost J, Karthaus WR, Gao D, Driehuis E, Sawyers CL, Chen Y, Clevers H. Organoid culture systems for prostate epithelial and cancer tissue. Nature protocols. 2016 Feb;11(2):347-58.

Huch M, Knoblich JA, Lutolf MP, Martinez-Arias A. The hope and the hype of organoid research. Development. 2017 Mar 15;144(6):938-41.

Liu L, Yu L, Li Z, Li W, Huang W. Patient-derived organoid (PDO) platforms to facilitate clinical decision making. Journal of Translational Medicine. 2021 Dec;19(1):1-9.

Fatehullah A, Tan SH, Barker N. Organoids as an in vitro model of human development and disease. Nature cell biology. 2016 Mar;18(3):246-54.

Kretzschmar K. Cancer research using organoid technology. Journal of Molecular Medicine. 2021 Apr;99(4):501-15.

Lancaster MA, Renner M, Martin CA, Wenzel D, Bicknell LS, Hurles ME, Homfray T, Penninger JM, Jackson AP, Knoblich JA. Cerebral organoids model human brain development and microcephaly. Nature. 2013 Sep;501(7467):373-9.

Greggio C, De Franceschi F, Figueiredo-Larsen M, Gobaa S, Ranga A, Semb H, Lutolf M, Grapin-Botton A. Artificial three-dimensional niches deconstruct pancreas development in vitro. Development. 2013 Nov 1;140(21):4452-62.

McCracken KW, Catá EM, Crawford CM, Sinagoga KL, Schumacher M, Rockich BE, Tsai YH, Mayhew CN, Spence JR, Zavros Y, Wells JM. Modelling human development and disease in pluripotent stem-cell-derived gastric organoids. Nature. 2014 Dec;516(7531):400-4.

Ciancanelli MJ, Huang SX, Luthra P, Garner H, Itan Y, Volpi S, Lafaille FG, Trouillet C, Schmolke M, Albrecht RA, Israelsson E. Life-threatening influenza and impaired interferon amplification in human IRF7 deficiency. Science. 2015 Apr 24;348(6233):448-53.

Fukuda M, Mizutani T, Mochizuki W, Matsumoto T, Nozaki K, Sakamaki Y, Ichinose S, Okada Y, Tanaka T, Watanabe M, Nakamura T. Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon. Genes & development. 2014 Aug 15;28(16):1752-7.

Dekkers JF, Wiegerinck CL, De Jonge HR, Bronsveld I, Janssens HM, De Winter-de Groot KM, Brandsma AM, De Jong NW, Bijvelds MJ, Scholte BJ, Nieuwenhuis EE. A functional CFTR assay using primary cystic fibrosis intestinal organoids. Nature medicine. 2013 Jul;19(7):939-45.

Takasato M, Er PX, Chiu HS, Maier B, Baillie GJ, Ferguson C, Parton RG, Wolvetang EJ, Roost MS, Chuva de Sousa Lopes SM, Little MH. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature. 2015 Oct;526(7574):564-8.

Spence JR, Mayhew CN, Rankin SA, Kuhar MF, Vallance JE, Tolle K, Hoskins EE, Kalinichenko VV, Wells SI, Zorn AM, Shroyer NF. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature. 2011 Feb;470(7332):105-9.

Wörsdörfer P, Asahina I, Sumita Y, Ergün S. Do not keep it simple: recent advances in the generation of complex organoids. Journal of Neural Transmission. 2020 Nov;127(11):1569-77.

Mekala SR, Wörsdörfer P, Bauer J, Stoll O, Wagner N, Reeh L, Loew K, Eckner G, Kwok CK, Wischmeyer E, Dickinson ME. Generation of cardiomyocytes from vascular adventitia-resident stem cells. Circulation Research. 2018 Aug 31;123(6):686-99.

Ormel PR, Vieira de Sá R, Van Bodegraven EJ, Karst H, Harschnitz O, Sneeboer MA, Johansen LE, van Dijk RE, Scheefhals N, Berdenis van Berlekom A, Ribes Martínez E. Microglia innately develop within cerebral organoids. Nature communications. 2018 Oct 9;9(1):1-4.

Wörsdörfer P, Dalda N, Kern A, Krüger S, Wagner N, Kwok CK, Henke E, Ergün S. Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells. Scientific reports. 2019 Oct 30;9(1):1-3.

Nabi SU, Ali SI, Rather MA, Sheikh WM, Altaf M, Singh H, Mumtaz PT, Mishra NC, Nazir SU, Bashir SM. Organoids: A new approach in toxicity testing of nanotherapeutics. Journal of Applied Toxicology. 2022 Jan;42(1):52-72.